Geldanamycin induces apoptosis in human gastric carcinomas by affecting multiple oncogenic kinases that have synergic effects with TNF-related apoptosis-inducing ligand

Oncology Letters
Hui ChenDun Pan

Abstract

The aim of the present study was to evaluate the effect of geldanamycin (GA) on the treatment of human gastric carcinomas and to investigate the molecular mechanism that provides the basis for the combination of GA with the tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induction strategy. The expression of target proteins at the mRNA level was determined using reverse transcription-polymerase chain reaction (RT-PCR), and apoptosis was evaluated with the terminal deoxynucleotidyl transferase mediated digoxigenin-dUTP nick-end labeling and Annexin V/propidium iodide (PI) staining methods. Phosphorylation of targeted kinases was studied using immunocytochemistry methods, and malignant phenotypes were studied using in vitro assays. GA treatment inhibits proliferation, migration and invasion, and induces apoptosis in human gastric cancer SGC-7901 cells, most likely by decreasing the expression of B-RAF and by phosphorylation of protein kinase B (AKT) and ERK. The inhibitory role of AKT in TRAIL regulation holds considerable potential for achieving a synergic effect in clinical therapy, using a combination of GA treatment and TRAIL induction. The present study provides a basis for the future application of hea...Continue Reading

References

Jun 19, 1992·International Journal of Cancer. Journal International Du Cancer·M FerrariniC Rugarli
Jan 1, 1995·Cancer Chemotherapy and Pharmacology·J G SupkoL Malspeis
Jan 5, 2002·Expert Opinion on Biological Therapy·Alison Maloney, Paul Workman
Oct 19, 2002·Biochimica Et Biophysica Acta·Luca M NeriAlberto M Martelli
May 30, 2003·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·David M Roder
Jul 26, 2003·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M P GoetzC Erlichman
May 29, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hong Zhang, Francis Burrows
Mar 11, 2005·Expert Opinion on Emerging Drugs·Len Neckers, Katharine Neckers
Jul 2, 2005·Expert Opinion on Emerging Drugs·Len Neckers, Katharine Neckers
Jul 7, 2005·Acta Biochimica Et Biophysica Sinica·Fei DouJing-Jing Zhu
Aug 9, 2005·Expert Opinion on Therapeutic Targets·Weiya Ma, Remi Quirion
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·O M GrbovicN Rosen
Jul 18, 2006·Current Topics in Medicinal Chemistry·David B Solit, Neal Rosen
Jul 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Fumitaka KogaLen Neckers
Sep 25, 2008·Nature Reviews. Cancer·Ricky W JohnstoneMark J Smyth
Apr 30, 2009·British Journal of Cancer·D MahalingamF J Giles
Sep 17, 2009·Journal of the National Cancer Institute·Karyn Hede
Oct 29, 2009·Current Topics in Medicinal Chemistry·Y S KimJ B Trepel
Jun 30, 2010·Digestive Diseases and Sciences·Maria J Redlak, Thomas A Miller
Nov 9, 2011·Biochimica Et Biophysica Acta·Komal JhaveriGabriela Chiosis
Aug 2, 2013·International Journal of Biological Sciences·Qingping MaWen Zhu
Dec 12, 2013·TheScientificWorldJournal·Ambuj KumarRituraj Purohit
Jul 9, 2014·Cancers·Tasuku Matsuoka, Masakazu Yashiro

❮ Previous
Next ❯

Citations

Jan 28, 2018·Cell Death & Disease·Tzu-Sheng HsuMing-Zong Lai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved