Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

Biomaterials Science
C R CarvalhoK Haastert-Talini

Abstract

Peripheral nerve injuries (PNI) resulting in a gap to be bridged between the transected nerve ends are commonly reconstructed with autologous nerve tissue, but there is a need for valuable alternatives. This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels made from chitosan with a 5% degree of acetylation. The engineered constructs should remodel the structural support given to regenerating axons by the so-called bands of Büngner. Four different GG formulations were produced by combining varying amounts of High-Acyl GG (HA-GG) and Methacrylated GG (MA-GG). The effective porosity of the freeze-dried networks was analysed by SEM and micro-CT 3D reconstructions, while the degradation and swelling abilities were characterized in vitro for up to 30 days. The metabolic activity and viability of immortalized Schwann cells seeded onto the freeze-dried networks were also evaluated. Finally, the developed hydrogel formulations were freeze-dried within the chitosan nerve guides and implanted in a 10 mm rat sciatic nerve defect. Functional and histomorphological analyses after 3, 6, and 12 weeks in vivo revealed that although it did not result in improved ner...Continue Reading

References

Jul 1, 1988·Journal of Reconstructive Microsurgery·G PenkertM Samii
Apr 10, 2004·Neurological Research·Jason S BelkasRajiv Midha
Jan 12, 2005·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Hitoshi NagatsukaNoriyuki Nagai
Dec 14, 2005·Journal of Biomedical Materials Research. Part a·Qiang AoXiufang Zhang
Dec 29, 2007·Seminars in Immunology·James M AndersonDavid T Chang
Apr 27, 2010·Biomaterials·Jason W NicholAli Khademhosseini
Jul 29, 2010·Biomaterials·Daniela F CoutinhoRui L Reis
Feb 1, 2011·Atlas of the Oral and Maxillofacial Surgery Clinics of North America·Martin B SteedRavi V Bellamkonda
May 24, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Haeyeon LeeChristopher J Hunter
May 24, 2011·Journal of Tissue Engineering and Regenerative Medicine·J Silva-CorreiaR L Reis
Aug 26, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Stefanie C HuelsenbeckKirsten Haastert-Talini
Sep 1, 2011·Journal of Neuroinflammation·Andrew D GaudetMatt S Ramer
Nov 1, 2011·Micron : the International Research and Review Journal for Microscopy·Ryuta Mizutani, Yoshio Suzuki
Dec 12, 2012·Journal of Tissue Engineering and Regenerative Medicine·G KhangR L Reis
Apr 10, 2013·Journal of Biomedical Materials Research. Part a·Joana Silva-CorreiaRui L Reis
Sep 21, 2013·Biomaterials·Kirsten Haastert-TaliniClaudia Grothe
Oct 8, 2013·International Review of Neurobiology·Sara GnaviStefano Geuna
Mar 13, 2014·Tissue Engineering. Part a·Sandra WrobelKirsten Haastert-Talini
May 14, 2014·Biomaterials·Xiaosong GuDavid F Williams
Jul 23, 2014·Acta Biomaterialia·Lucília P da SilvaAlexandra P Marques
Oct 3, 2014·BioMed Research International·D Grinsell, C P Keating
Dec 2, 2014·Tissue Engineering. Part a·Atefeh MobasseriAdam J Reid
Dec 3, 2014·Advanced Drug Delivery Reviews·Alessandro FaroniAdam J Reid
Dec 3, 2014·Journal of the Mechanical Behavior of Biomedical Materials·T M DinisF Marin
Dec 3, 2014·Journal of the Mechanical Behavior of Biomedical Materials·Rukmani SridharanConor T Buckley
Mar 6, 2015·Frontiers in Cellular Neuroscience·Oscar A Carballo-Molina, Iván Velasco
Jul 16, 2015·Journal of Tissue Engineering and Regenerative Medicine·Timothy E L DouglasElzbieta Pamula
Oct 16, 2015·Tissue Engineering. Part a·Laura M MarquardtShelly E Sakiyama-Elbert
Dec 1, 2015·Drug Development and Industrial Pharmacy·Fernanda Isadora BoniBeatriz Stringhetti Ferreira Cury
Apr 5, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Sunil Kumar VimalDhirendra S Katti
Jul 19, 2016·Biomaterials Science·L R StevensM In Het Panhuis

❮ Previous
Next ❯

Citations

Sep 28, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Zhen ZhangChenping Zhang
Jan 24, 2019·Biomaterials Science·Shiwha ParkKyung Jae Jeong
Feb 15, 2020·Macromolecular Bioscience·Deteng ZhangChangyou Gao
Apr 16, 2020·Journal of Tissue Engineering and Regenerative Medicine·Jesús Chato-AstrainVictor Carriel
Dec 12, 2019·Frontiers in Bioengineering and Biotechnology·Cristiana R CarvalhoRui L Reis
Nov 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuqing Niu, Massimiliano Galluzzi

❮ Previous
Next ❯

Methods Mentioned

BETA
xenografts
Acetylation
X-ray
Electrophoresis

Software Mentioned

CellSense Dimension
NRecon
cellP
DataViewer
CT Analyser
GraphPad
AUTOGs
μCT Evaluation Program
CellSense Entry
AUTOG

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.