Gelsolin affects the migratory ability of human colon adenocarcinoma and melanoma cells

Life Sciences
Monika LitwinM Malicka-Błaszkiewicz

Abstract

Formation of different protrusive structures by migrating cells is driven by actin polymerization at the plasma membrane region. Gelsolin is an actin binding protein controlling the length of actin filaments by its severing and capping activity. The main goal of this study was to determine the effect of gelsolin expression on the migration of human colon adenocarcinoma LS180 and melanoma A375 cells. Colon adenocarcinoma cell line LS180 was stably transfected with plasmid containing human cytoplasmic gelsolin cDNA tagged to enhanced green fluorescence protein (EGFP). Melanoma A375 cells were transfected with siRNAs directed against gelsolin. Real-time PCR and Western blotting were used to determine the level of gelsolin. The ability of actin to inhibit DNase I activity was used to quantify monomeric and total actin level and calculate the state of actin polymerization. Fluorescence confocal microscopy was applied to observe gelsolin and vinculin distribution along with actin cytoskeleton organization. Increased level of gelsolin expression leads to its accumulation at the submembranous region of the cell accompanied by distinct changes in the state of actin polymerization and an increase in the migration of LS180 cells. In addit...Continue Reading

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Citations

Jul 22, 2015·The FEBS Journal·Zheng QuHans Georg Mannherz
Sep 30, 2016·International Journal of Cancer. Journal International Du Cancer·Kaname OhyamaNaotaka Kuroda
Feb 18, 2017·International Journal of Molecular Sciences·Lei ZhangXiaoming Jin
Oct 21, 2015·Journal of Biomedical Science·Zhi-Yuan ChenYing-Ming Liou
May 30, 2013·Journal of Proteome Research·He-chun LinMing Yao
Oct 12, 2021·Frontiers in Oncology·Nicole Elisabeth TopalovBastian Czogalla

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