Gemcitabine induces programmed cell death and activates protein kinase C in BG-1 human ovarian cancer cells

Cancer Chemotherapy and Pharmacology
L Cartee, G L Kucera

Abstract

Cytosine arabinoside induces apoptosis and this cell death process is influenced by protein kinase C signaling events in leukemic cells. We present findings that extend these observations to include another deoxycytidine analog, gemcitabine, which is more potent in solid tumors. Gemcitabine induced programmed cell death in BG-1 human ovarian cancer cells based on biochemical and morphologic analyses. The DNA was fragmented in BG-1 cells exposed to gemcitabine (0.5 microM, 1.0 microM and 10 microM) for 8 h, but gemcitabine treatment did not induce internucleosomal DNA degradation. Scanning and transmission electron microscopy of BG-1 cells showed morphologic changes associated with apoptosis in response to gemcitabine: membrane blebbing, the formation of apoptotic bodies and chromatin condensation. Thus, BG-1 cells undergo programmed cell death in response to gemcitabine treatment without internucleosomal DNA fragmentation. Furthermore, gemcitabine (10 microM) activated protein kinase C in BG-1 cells and the phosphorylation of the endogenous protein kinase C substrate, myristoylated alanine-rich C kinase substrate, was increased following exposure of BG-1 cells to gemcitabine for up to 6 h. Clonogenicity studies with gemcitabine...Continue Reading

Citations

Jul 20, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Andries M BergmanGodefridus J Peters
Mar 12, 2004·Anti-cancer Drugs·Thomas SchöndorfChristian M Kurbacher
Dec 25, 2013·International Journal of Oncology·Hong-Quan DuongInsoo Bae
Dec 7, 2000·Cancer Investigation·L Cartee, G L Kucera
Jan 30, 2004·International Journal of Cancer. Journal International Du Cancer·Bodo SchniewindHendrik Ungefroren
Aug 18, 2004·Biochemical Pharmacology·Liron HirshAbraham Amsterdam
May 24, 2006·Seminars in Oncology·Sergio PecorelliFranco Odicino
Jan 7, 2014·Trends in Pharmacological Sciences·Theresa M FiltzMark Leid
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna K NowakBruce W S Robinson
Sep 21, 2006·Molecular Cancer Therapeutics·Anshu M RoyZhican Qu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis