Gemin5 proteolysis reveals a novel motif to identify L protease targets.

Nucleic Acids Research
David PiñeiroEncarnación Martínez-Salas

Abstract

Translation of picornavirus RNA is governed by the internal ribosome entry site (IRES) element, directing the synthesis of a single polyprotein. Processing of the polyprotein is performed by viral proteases that also recognize as substrates host factors. Among these substrates are translation initiation factors and RNA-binding proteins whose cleavage is responsible for inactivation of cellular gene expression. Foot-and-mouth disease virus (FMDV) encodes two proteases, L(pro) and 3C(pro). Widespread definition of L(pro) targets suffers from the lack of a sufficient number of characterized substrates. Here, we report the proteolysis of the IRES-binding protein Gemin5 in FMDV-infected cells, but not in cells infected by other picornaviruses. Proteolysis was specifically associated with expression of L(pro), yielding two stable products, p85 and p57. In silico search of putative L targets within Gemin5 identified two sequences whose potential recognition was in agreement with proteolysis products observed in infected cells. Mutational analysis revealed a novel L(pro) target sequence that included the RKAR motif. Confirming this result, the Fas-ligand Daxx, was proteolysed in FMDV-infected and L(pro)-expressing cells. This protein c...Continue Reading

References

Dec 23, 2000·The Journal of General Virology·M SáizF Sobrino
Nov 21, 2001·The Journal of Biological Chemistry·Amelie K GubitzGideon Dreyfuss
Oct 18, 2002·Nucleic Acids Research·Sonia López de QuintoEncarnación Martínez-Salas
Dec 17, 2002·Journal of Cell Science·Jennifer S Michaelson, Philip Leder
Oct 17, 2003·RNA·Olga Fernández-Miragall, Encarnación Martínez-Salas
Apr 23, 2005·The Journal of Biological Chemistry·Nickolay NeznanovAndrei V Gudkov
May 18, 2006·Molecular and Cellular Biology·Mi-Young JungJoel D Richter
Jul 22, 2006·Molecular Cell·Daniel J BattleGideon Dreyfuss
Sep 8, 2006·RNA·Stephen D BairdMartin Holcik
Jan 17, 2007·Nature Reviews. Microbiology·Laurent BalvayThéophile Ohlmann
Apr 21, 2007·Virology·Miguel Rodríguez PulidoEncarnación Martínez-Salas
May 3, 2007·Genes & Development·Laura L Almstead, Peter Sarnow
Jun 22, 2007·Journal of Virology·Rushika PereraBert L Semler
Jul 21, 2007·BMC Cell Biology·Le thi HaoGlenn E Morris
Sep 21, 2007·Journal of Virology·Teresa de Los SantosMarvin J Grubman
Nov 17, 2007·Cell Host & Microbe·James P WhiteRichard E Lloyd
Feb 15, 2008·The Journal of General Virology·Encarnación Martínez-SalasNoemi Fernandez
Apr 19, 2008·Trends in Microbiology·Encarnación Martínez-Salas
Jun 24, 2008·Virology·Sylvain de BreyneChristopher U T Hellen
Jul 22, 2008·Virus Research·Peter J Lukavsky
Dec 11, 2008·Nucleic Acids Research·Almudena PachecoEncarnación Martínez-Salas
Apr 14, 2009·Current Opinion in Structural Biology·Megan E Filbin, Jeffrey S Kieft
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sylvain de BreyneChristopher U T Hellen
Jul 25, 2009·Virology·Paola M BarralVincent R Racaniello
Jul 28, 2009·Biochimica Et Biophysica Acta·Kerry D Fitzgerald, Bert L Semler
Sep 15, 2009·PloS One·Shelton S Bradrick, Matthias Gromeier

❮ Previous
Next ❯

Citations

Dec 12, 2012·Nucleic Acids Research·David PiñeiroEncarnación Martínez-Salas
Jul 21, 2012·PloS One·Teodoro FajardoEncarnacion Martinez-Salas
Nov 6, 2013·International Journal of Molecular Sciences·Encarnación Martínez-SalasRosa Diaz
Mar 7, 2014·Nucleic Acids Research·Javier Fernandez-ChamorroEncarnación Martinez-Salas
Jan 27, 2015·Virus Research·Encarnación Martínez-SalasRosa Diaz-Toledano
May 26, 2015·Current Opinion in Virology·Gloria Lozano, Encarnación Martínez-Salas
Apr 22, 2015·Biomolecules·David PiñeiroEncarna Martinez-Salas
Jun 30, 2018·PLoS Pathogens·Miguel Rodríguez PulidoMargarita Sáiz
Jul 30, 2017·The FEBS Journal·Alfonso GalanEncarnacion Martinez-Salas
May 12, 2017·Journal of Virology·Pengcheng ShangYing Fang
Mar 29, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Rosario Francisco-VelillaEncarnacion Martinez-Salas
Jun 2, 2020·RNA Biology·Rosario Francisco-VelillaEncarnacion Martinez-Salas
Nov 30, 2018·Frontiers in Microbiology·Gisselle N MedinaTeresa de Los Santos
Dec 5, 2019·Nucleic Acids Research·María Moreno-MorcilloEncarnacion Martinez-Salas
Jun 4, 2020·International Journal of Molecular Sciences·Encarnacion Martinez-SalasRosario Francisco-Velilla
Jan 23, 2018·Frontiers in Microbiology·Encarnacion Martinez-SalasAzman M Embarek
Sep 26, 2019·Frontiers in Microbiology·Di SunXiaoyue Chen
Feb 20, 2021·Wiley Interdisciplinary Reviews. RNA·Margarita Saiz, Encarnacion Martinez-Salas
Jun 3, 2021·Viruses·Azman Embarc-BuhEncarnacion Martinez-Salas

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.