Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis

Osteoarthritis and Cartilage
E KriegovaJ Gallo

Abstract

There is no existing comprehensive report on the cellular composition of synovial fluids (SFs) from knee osteoarthritis (OA). We therefore aimed to characterise the immune cell composition in SFs from knee OA (KOA) and in subgroups according to gender. The immunophenotyping of monocyte/macrophage lineage cells, T and B cells, NK cells, neutrophils, dendritic and mast cells (MC) present in SFs from 53 patients (24 males/29 females) with KOA was performed using 6-colour flow cytometry. SFs from patients with OA contained 90% hematopoietic cells. Lymphocytes were the predominant cell population (44.8%) in the SFs of OA patients, with CD4+ T lymphocytes being more prevalent than CD8+ T cells (CD4+/CD8+ ratio = 1.3). Within the monocyte/macrophage lineage gating, monocytes accounted for 33.9%, macrophages 14.8%, myeloid dendritic cells 16.4%. The rest of the hematopoietic cells were comprised of neutrophils (8%), NK cells (3.8%), T regulatory cells (1.2%), plasmacytoid dendritic cells (1.1%), mast cells (0.3%). In OA females, a higher percentage of CD4+ T cells (P = 0.023), macrophages (P = 0.012), and a lower percentage of monocytes (P = 0.008) and CD8+ T cells (P = 0.002) were detected in comparison to OA males. Based on the immun...Continue Reading

Citations

Jan 13, 2021·International Journal of Molecular Sciences·Chenshuang Li, Zhong Zheng
Jan 27, 2020·Osteoarthritis and Cartilage·H ZhangX Bai
Aug 8, 2021·International Journal of Molecular Sciences·Chenshuang Li, Zhong Zheng
Sep 9, 2021·Journal of Experimental Orthopaedics·Junpei DanMasahiko Ikeuchi
Nov 6, 2021·Arthritis Research & Therapy·Sophia Y Kim-WangAmy L McNulty

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