Gene 5.5 protein of bacteriophage T7 in complex with Escherichia coli nucleoid protein H-NS and transfer RNA masks transfer RNA priming in T7 DNA replication.

Proceedings of the National Academy of Sciences of the United States of America
Bin ZhuC C Richardson

Abstract

DNA primases provide oligoribonucleotides for DNA polymerase to initiate lagging strand synthesis. A deficiency in the primase of bacteriophage T7 to synthesize primers can be overcome by genetic alterations that decrease the expression of T7 gene 5.5, suggesting an alternative mechanism to prime DNA synthesis. The product of gene 5.5 (gp5.5) forms a stable complex with the Escherichia coli histone-like protein H-NS and transfer RNAs (tRNAs). The 3'-terminal sequence (5'-ACCA-3') of tRNAs is identical to that of a functional primer synthesized by T7 primase. Mutations in T7 that suppress the inability of primase reduce the amount of gp5.5 and thus increase the pool of tRNA to serve as primers. Alterations in T7 gene 3 facilitate tRNA priming by reducing its endonuclease activity that cleaves at the tRNA-DNA junction. The tRNA bound to gp5.5 recruits H-NS. H-NS alone inhibits reactions involved in DNA replication, but the binding to gp5.5-tRNA complex abolishes this inhibition.

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Citations

Nov 29, 2013·Nucleic Acids Research·Bin ZhuCharles C Richardson
Jun 4, 2015·Annual Review of Biochemistry·Charles C Richardson
Feb 2, 2015·Current Opinion in Microbiology·Robert LandickDavid C Grainger
Jul 23, 2016·Frontiers in Microbiology·Sana HamdiKarim B Slama
Dec 14, 2016·Nucleic Acids Research·Isao MasudaYa-Ming Hou
Jan 1, 2016·The Behavioral and Brain Sciences·Ezequiel MorsellaAdam Gazzaley
Jan 8, 2014·The Journal of Biological Chemistry·Hitoshi MitsunobuCharles C Richardson
May 10, 2017·Nucleic Acids Research·Aline Tabib-SalazarSivaramesh Wigneshweraraj
Sep 15, 2021·Nucleic Acids Research·Fredj Ben BdiraRemus T Dame
Aug 26, 2020·Annual Review of Genetics·Zhangli SuAnindya Dutta

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