Gene amplification of the Hps locus in Glycine max

BMC Plant Biology
M GijzenPat Moy

Abstract

Hydrophobic protein from soybean (HPS) is an 8 kD cysteine-rich polypeptide that causes asthma in persons allergic to soybean dust. HPS is synthesized in the pod endocarp and deposited on the seed surface during development. Past evidence suggests that the protein may mediate the adherence or dehiscence of endocarp tissues during maturation and affect the lustre, or glossiness of the seed surface. A comparison of soybean germplasm by genomic DNA blot hybridization shows that the copy number and structure of the Hps locus is polymorphic among soybean cultivars and related species. Changes in Hps gene copy number were also detected by comparative genomic DNA hybridization using cDNA microarrays. The Hps copy number polymorphisms co-segregated with seed lustre phenotype and HPS surface protein in a cross between dull- and shiny-seeded soybeans. In soybean cultivar Harosoy 63, a minimum of 27 +/- 5 copies of the Hps gene were estimated to be present in each haploid genome. The isolation and analysis of genomic clones indicates that the core Hps locus is comprised of a tandem array of reiterated units, with each 8.6 kb unit containing a single HPS open reading frame. This study shows that polymorphisms at the Hps locus arise from ch...Continue Reading

References

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Citations

Feb 27, 2010·Planta·Kosala RanathungeMark A Bernards
May 8, 2014·International Journal of Genomics·Bhaskar Gupta, Bingru Huang
Aug 30, 2014·Microbiology·Wannasiri WannaratTakashi Inaoka
Aug 10, 2007·Critical Reviews in Biochemistry and Molecular Biology·P J Hastings
Jul 21, 2017·The Plant Genome·Nonoy B BandilloJames E Specht
Sep 26, 2006·Trends in Microbiology·Dinah QutobMark Gijzen
May 20, 2014·Journal of Agricultural and Food Chemistry·Krishna KuppannanBarry Schafer

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Datasets Mentioned

BETA
GSE3810

Methods Mentioned

BETA
PCR
electrophoresis
gel filtration

Software Mentioned

Lasergene
Array Vision

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