Gene array of primary human osteoblasts exposed to enamel matrix derivative in combination with a natural bone mineral.

Clinical Oral Investigations
Richard J MironA Sculean

Abstract

The application of an enamel matrix derivative (EMD) for regenerative periodontal surgery has been shown to promote formation of new cementum, periodontal ligament, and alveolar bone. In intrabony defects with a complicated anatomy, the combination of EMD with various bone grafting materials has resulted in additional clinical improvements, but the initial cellular response of osteoblasts coming in contact with these particles have not yet been fully elucidated. The objective of the present study was to evaluate the in vitro effects of EMD combined with a natural bone mineral (NBM) on a wide variety of genes, cytokines, and transcription factors and extracellular matrix proteins on primary human osteoblasts. Primary human osteoblasts were seeded on NBM particles pre-coated with versus without EMD and analyzed for gene differences using a human osteogenesis gene super-array (Applied Biosystems). Osteoblast-related genes include those transcribed during bone mineralization, ossification, bone metabolism, cell growth and differentiation, as well as gene products representing extracellular matrix molecules, transcription factors, and cell adhesion molecules. EMD promoted gene expression of various osteoblast differentiation markers...Continue Reading

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Citations

Apr 14, 2015·Periodontology 2000·Miron Weinreb, Carlos E Nemcovsky
May 27, 2014·International Journal of Molecular Sciences·Elisabeth Aurstad RiksenJanne E Reseland
Oct 21, 2016·Clinical Oral Investigations·Richard J MironYufeng Zhang
Jan 8, 2014·Clinical Implant Dentistry and Related Research·Shuang YangYufeng Zhang
Dec 19, 2014·Clinical Oral Investigations·Richard J MironAnton Sculean
Jul 16, 2015·Clinical Oral Investigations·Yufeng ZhangRichard J Miron
Mar 4, 2015·Biochimica Et Biophysica Acta·Divya Ramchandani, Georg F Weber
May 9, 2014·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·L WeiY Zhang
Mar 19, 2016·Clinical Oral Implants Research·Masako Fujioka-KobayashiRichard J Miron
Oct 21, 2016·Clinical Oral Investigations·Richard J MironYufeng Zhang
May 12, 2018·International Journal of Oral Science·Sema S HakkiWerner Götz

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