PMID: 20626138Jul 16, 2010Paper

Gene chips-aided analysis on the profiles of hippocampal whole-genome expression in depression rats following electroacupuncture treatment

Zhen ci yan jiu = Acupuncture research
Li-hua Jiang, Ling-ling Wang

Abstract

To observe the effect of electroacupuncture (EA) on the profiles of hippocampal whole-genome expression in depression rats so as to study its underlying mechanism in relieving depression. Thirty male SD rats were randomized into normal, model and EA groups (n = 10/group). Depression model was established by chronic unpredictable stress stimulation (forced ice-water swimming, electric shock, tail-clamping, etc.) and lonely raising for 21 days. EA (2 Hz, 1 mA) was applied to "Baihui"(GV 20) and "Yintang" (EX-HN 3) for 15 min, once daily for 7 days. The rats' ethological activities (horizontal and vertical movements) were detected with Open-field test in Morris labyrinth, and 1% sucrose solution consumption was detected. After termination of the treatment, the rats were killed and their hippocampal tissues were taken out for analyzing the whole-genome expression with Roche-NimbleGen chips (Rattus norvegicus 12 x 135 Karray). Compared with normal group, the distance of horizontal movement, rearing times, and sucrose consumption at day 21 and day 28 of model group decreased considerably (P < 0.01). In comparison with model group, the sucrose consumption at day 28 in EA group increased significantly (P < 0.05). Results of gene expres...Continue Reading

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