PMID: 8946382Nov 25, 1996Paper

Gene cloning and cellular localization of a membrane-bound acid phosphatase of Leishmania mexicana

Molecular and Biochemical Parasitology
M WieseP Overath

Abstract

In a previous publication, we described the purification of a membrane-bound acid phosphatase of Leishmania mexicana as a heterogeneously N-glycosylated protein of an apparent molecular mass of 70000-72000 expressed in both the promastigote and the amastigote stage of the parasite [19]. Screening of a genomic DNA library of L. mexicana with degenerate oligonucleotides designed according to the NH2-terminus of the protein led to the cloning of the lmmbap gene, which is present in one copy per haploid genome. The open reading frame predicts a protein of 516 amino acids composed of a signal sequence, a large hydrophilic region, a trans-membrane alpha-helix and a short cytoplasmic tail. The sequence of the hydrophilic region is homologous to acid phosphatases from other organisms. While in wild-type promastigotes, the acid phosphatase is located in the endosomal/lysosomal compartment between the flagellar pocket and the nucleus, overexpression leads to its abundant exposure on the cell surface. In cells transfected with a construct lacking the region corresponding to the trans-membrane and the cytoplasmic parts, the resulting altered acid phosphatase is efficiently secreted into the culture medium. The potential of this system for ...Continue Reading

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Citations

Oct 24, 2002·International Journal for Parasitology·Ross F Waller, Malcolm J McConville
Nov 26, 2003·International Journal for Parasitology·Martin WieseIris Görcke
Nov 23, 2000·Molecular and Biochemical Parasitology·I BenzelM Wiese
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Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Malcolm J McConvilleRohan D Teasdale
May 24, 2011·Journal of Parasitology Research·Marta T GomesJosé Roberto Meyer-Fernandes
Jun 26, 2012·Trends in Parasitology·Jan M BoitzNicola S Carter
Apr 25, 2009·Free Radical Biology & Medicine·Daniela Cosentino-GomesJosé Roberto Meyer-Fernandes
Jul 4, 2006·Experimental Parasitology·Elmo Eduardo de Almeida-AmaralJosé Roberto Meyer-Fernandes
Dec 21, 2005·Micron : the International Research and Review Journal for Microscopy·Luciana LopesDorly de Freitas Buchi
Feb 20, 2015·The Biochemical Journal·Amalia PapadakiHaralabia Boleti
Jul 1, 2011·Veterinary Parasitology·Ping ZhangHui Shen
Jan 22, 2011·Journal of Bioenergetics and Biomembranes·Daniela Cosentino-Gomes, José Roberto Meyer-Fernandes
Dec 21, 2007·The Biochemical Journal·Daniel J Rigden
May 11, 2021·Frontiers in Cellular and Infection Microbiology·Anita Leocadio Freitas-MesquitaJosé Roberto Meyer-Fernandes

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