Gene cloning and functional characterization of four novel antimicrobial-like peptides from scorpions of the family Vaejovidae

Peptides
Santos Ramírez-CarretoErnesto Ortiz

Abstract

From the cDNA libraries made from the venom glands of two scorpions belonging to the Vaejovidae family, four different putative non disulfide-bridged antimicrobial peptides were identified: VmCT1 and VmCT2 from Vaejovis mexicanus smithi plus VsCT1 and VsCT2 from Vaejovis subcristatus. These short peptides (with only 13 amino acid residues each) share important amino acid sequence similarities among themselves and with other reported antimicrobial peptides, but their biological activities vary dramatically. This communication reports the cloning, chemical synthesis and characterization of these peptides. Two peptides, VmCT1 and VmCT2 showed broad-spectrum antibacterial activity with minimum inhibitory concentrations MICs in the range of 5-25 μM and 10-20 μM respectively, whereas their hemolytic activity at these concentrations was low. Structure-function relationships that might determine the differences in activities are discussed.

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Citations

Jul 12, 2013·Journal of Toxicology·Bennasr HmedZeghal Khaled Mounir
Jun 22, 2014·Toxicon : Official Journal of the International Society on Toxinology·Patrick L HarrisonPeter N Strong
Dec 30, 2015·Toxins·Carlos E Santibáñez-LópezLourival D Possani
Jul 16, 2014·Toxins·Sebastián Estrada-GómezLeidy Johana Vargas Muñoz
Jul 15, 2015·Toxicon : Official Journal of the International Society on Toxinology·Rodolfo Rodríguez-RaveloLourival D Possani
Sep 10, 2015·Peptides·Santos Ramírez-CarretoErnesto Ortiz
Oct 20, 2015·Acta Tropica·Sebastián Estrada-GómezJuan Carlos Quintana Castillo
Nov 28, 2012·Toxicon : Official Journal of the International Society on Toxinology·Karen Luna-RamírezLourival D Possani
Feb 12, 2014·Peptides·Rodolfo Rodríguez-RaveloLourival D Possani
Nov 5, 2013·Peptides·Ammar Almaaytah, Qosay Albalas
Apr 30, 2013·Peptides·Xian-Chun ZengHanjun Cao
Nov 29, 2014·Toxicon : Official Journal of the International Society on Toxinology·Ernesto OrtizLourival D Possani
Feb 7, 2015·PloS One·Verónica Quintero-HernándezErnesto Ortiz
Dec 13, 2017·Toxins·Teresa Romero-GutierrezLourival D Possani
Oct 19, 2017·Chemical Biology & Drug Design·Cibele Nicolaski PedronVani Xavier Oliveira
Jun 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea Díaz-RoaFelio J Bello
Jun 24, 2018·Pharmaceutics·Ilaria PassariniMire Zloh
Oct 3, 2020·Parasitology·Cibele Nicolaski PedronVani Xavier Oliveira
Jan 6, 2021·Journal of Biomolecular Structure & Dynamics·José-Luis Velasco-Bolom, Ramón Garduño-Juárez
Jan 15, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Cibele Nicolaski PedronVani Xavier Oliveira Junior
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Jul 17, 2018·Toxicon : Official Journal of the International Society on Toxinology·Micaiah J WardGunnar S Nystrom
Jul 23, 2021·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Sebastian Estrada-GómezArie van der Meijden
Aug 10, 2020·Mini Reviews in Medicinal Chemistry·Zhi-Gang SunXin Ming

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