Gene cloning, characterization, and heterologous expression of levansucrase from Bacillus amyloliquefaciens.

Journal of Industrial Microbiology & Biotechnology
Dina RairakhwadaChul Ho Kim

Abstract

Although levan produced by Bacillus amyloliquefaciens is known to have efficient immunostimulant property which gives 100% survival of common carp when infected with Aeromonas hydrophila, no detailed reports are available describing kinetic studies of D: -glucose production and levan formation. In this study, we cloned and characterized the enzymatic kinetics using levansucrase expressed in Escherichia coli. Optimum pH for D: -glucose production and levan formation was 6.0 and 8.0, respectively, whereas optimum temperature was 30 degrees C and 4 degrees C, respectively. The K (m) and V (max) values for levansucrase were calculated to be 47.81 mM sucrose and 57.47 1mole/min mg protein, respectively. Prominent expression of levansucrase was obtained through xylose induction in Bacillus megaterium, where most of the His(6)-tagged protein was secreted into the culture broth, giving levansucrase activity of 12,906 U/l. Response-surface methodology (RSM) was further employed to optimize the fermentation conditions and improve the level of levansucrase production. Maximum levansucrase activity of 20,251 U/l was obtained in 12 h of fermentation carried out at 28 degrees C, starting induction with 0.735% xylose when A (600) was 1.2, whi...Continue Reading

References

Mar 1, 1995·Applied Microbiology and Biotechnology·K D Wittchen, F Meinhardt
May 1, 1994·Microbiology·P S Vary
Sep 15, 1995·Structure·G Davies, B Henrissat
Aug 28, 1998·Applied Microbiology and Biotechnology·H BieblW D Deckwer
Feb 3, 1999·Annals of the New York Academy of Sciences·K B SongS K Rhee
Aug 15, 2003·The Journal of Biological Chemistry·Helen K LeechMartin J Warren
Mar 31, 2004·Canadian Journal of Microbiology·Marcus SchallmeyOwen P Ward
Jan 18, 2005·Biochimica Et Biophysica Acta·Hee Kyoung KangJohn F Robyt
Jan 25, 2007·Applied Microbiology and Biotechnology·Rebekka BiedendieckDieter Jahn

❮ Previous
Next ❯

Citations

Apr 14, 2011·The Protein Journal·Arundhati PaulRatan Gachhui
Jul 15, 2015·Applied Microbiology and Biotechnology·Wenjing LiWanmeng Mu
Dec 5, 2013·Carbohydrate Polymers·Tao ZhangBo Jiang
Jul 28, 2019·World Journal of Microbiology & Biotechnology·Shan CaoYu Li
Apr 30, 2017·Journal of Agricultural and Food Chemistry·Wei XuWanmeng Mu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.