Jun 7, 2014

mir-21 is associated with inactive low molecular weight Argonaute complexes in thyroid cancer cell lines

BioRxiv : the Preprint Server for Biology
David C PlachetzkiKenneth W. Witwer


Thyroid cancer is the most prevalent malignancy of the endocrine system. We and others have shown that several microRNAs, which are post-transcriptional gene regulators, are aberrantly expressed in anaplastic thyroid cancer (ATC) and papillary thyroid cancer (PTC) tissues, as well as cell lines derived from these cancers. In the cell, miRNAs are bound to Argonaute (AGO) proteins as what could be termed low molecular weight RNA-Induced Silencing Complexes (LMW-RISCs) that can then assemble with additional proteins, mRNA, and translation machinery into high molecular weight RISCs (HMW-RISCs) that also exert regulatory function. In this study, we sought to analyze the association of miRNAs with RISC complexes in ATC and PTC. For ATC and PTC lines, miRNA species were enriched in both HMW-RISC and LMW-RISC cellular fractions, compared with intermediate molecular weight fractions and very low molecular weight (AGO-poor) fractions. Furthermore, 60% of all miRNAs were slightly more abundant in LMW-RISC versus HMW-RISC fractions by ~2-4 fold. Surprisingly, miR-21-5p, one of the most abundant miRNAs in both ATC and PTC lines and one of the most widely studied oncogenic miRNAs in many solid tumors, was consistently one the least abundant ...Continue Reading

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Mentioned in this Paper

Gene Polymorphism
Cnox-2 protein, Hydractinia symbiolongicarpu
RNA library
Sequence Determinations, RNA
Nucleic Acid Sequencing
Gene Expression

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