Gene delivery efficiency and cytotoxicity of heterocyclic amine-modified PAMAM and PPI dendrimers

Materials Science & Engineering. C, Materials for Biological Applications
Maryam HashemiMohammad Ramezani

Abstract

Poly-(amidoamine) (PAMAM) and poly-(propylenimine) (PPI) are the two most widely investigated dendrimers for drug and gene delivery. In order to enhance DNA transfection activity of these dendrimers, generation 3 and 4 PAMAM and generation 4 and 5 PPI were modified by partial substitution of surface primary amines with histidine, pyridine, and piperazine, which have buffering capacity properties. It was shown that higher dendrimer generations and higher grafting percentages (30% and 50% of primary amines) were associated with higher transfection activity. Pyridine was the most effective substituent for PPI, while piperazine-modified PAMAM dendrimers showed the best transfection efficiency among PAMAM-based vectors in murine neuroblastoma (Neuro-2a) cells. None of the modified carriers showed remarkable cytotoxicity in vitro. Pretreatment of cells with bafilomycin A indicated that endosomal buffering capacity is the main mechanism of endosomal escape. In conclusion, PAMAM and PPI may exhibit different gene delivery efficiency and cytotoxicity profiles with the same chemical modifications. These modified dendrimers could be considered as efficient and safe gene carriers in neuroblastoma cells in vitro.

References

Sep 29, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joon Sig ChoiJong-Sang Park
Nov 29, 2005·Advanced Drug Delivery Reviews·Christine DufèsAndreas G Schätzlein
Mar 2, 2006·Pharmacological Reviews·Ikramy A KhalilHideyoshi Harashima
Aug 15, 2006·Biomacromolecules·Nathan P Gabrielson, Daniel W Pack
Dec 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Leto-Aikaterini TzivelekaConstantinos M Paleos
Apr 19, 2007·Drug News & Perspectives·Qixin LengA James Mixson
Sep 28, 2007·Current Pharmaceutical Design·H S Parekh
Oct 16, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shyh-Dar Li, Leaf Huang
Sep 25, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Verena RussErnst Wagner
Nov 26, 2008·Bioorganic & Medicinal Chemistry Letters·Rafique Ul IslamPatrick Arbuthnot
Aug 4, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Basavaraj K NanjwadeVeerendra K Nanjwade
Feb 9, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·José L SantosHelena Tomás
May 27, 2010·Bioconjugate Chemistry·Kai-Ling ChangMitsuru Hashida
Aug 3, 2010·Current Opinion in Biotechnology·Chantal PichonPatrick Midoux
Oct 12, 2010·Cancer Gene Therapy·M HashemiM Ramezani
Feb 26, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Neha ShahHarendra S Parekh
Oct 25, 2011·Chemical Communications : Chem Comm·Emilie BertrandPhilippe Guégan
Nov 6, 2012·Carbohydrate Polymers·Warayuth SajomsangSatit Puttipipatkhachorn
Nov 21, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Wen-Chi TsengTsuei-Yun Fang
Mar 19, 2013·Journal of the American Chemical Society·Hanxiang ZengZhibin Guan
Jul 26, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Zahra SalmasiMohammad Ramezani

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Citations

Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Vesta D ShevchenkoTimur I Abdullin
May 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Francesca SavianoClaudia Conte
Jun 30, 2016·Nanomedicine·Abhay Pandit, Dimitrios I Zeugolis
Jun 13, 2020·International Journal of Molecular Sciences·Sylwia MichlewskaMaria Bryszewska
Jul 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nadezhda N ShevelevaErkki Lahderanta
Mar 26, 2016·Polymers·Pablo Mesa-AntunezEzequiel Perez-Inestrosa

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