Gene delivery methods and genome editing of human pluripotent stem cells

Reports of Practical Oncology and Radiotherapy : Journal of Greatpoland Cancer Center in Poznań and Polish Society of Radiation Oncology
Patrycja CzerwińskaMaciej Wiznerowicz

Abstract

Induced pluripotent stem cells derived from normal somatic cells could be utilized to study tumorigenesis through overexpression of specific oncogenes, downregulation of tumor suppressors and dysregulation of other factors thought to promote tumorigenesis. Therefore, effective approaches that provide direct modifications of induced pluripotent stem cell genome are extremely needed. Emerging strategies are expected to provide the ability to more effectively introduce diverse genetic alterations, from as small as single-nucleotide modifications to whole gene amplification or deletion, all with a high degree of target specificity. To date, several techniques have been applied in stem cell studies to directly edit cell genome (ZFNs, TALENs or CRISPR/Cas9). In this review, we summarize specific gene delivery strategies that were applied to stem cell studies together with genome editing techniques, which enable a direct modification of endogenous DNA sequences in the context of cancer studies.

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Citations

Apr 18, 2020·Stem Cells International·Israa Ahmed RadwanKarim M Fawzy El-Sayed
Nov 18, 2020·Stem Cell Research & Therapy·Mohsen EbrahimiMaryam Farzaneh
May 1, 2021·Pharmaceuticals·Megan A YamoahXiao-Dong Zhang

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