Gene delivery to dendritic cells facilitated by a tumor necrosis factor alpha-competing peptide

Molecular Immunology
Sai P ChamarthyWilson S Meng

Abstract

Efficient gene delivery systems tailor-designed for dendritic cells (DCs) would allow the possibility of therapeutic manipulation of a wide spectrum of immune functions. Toward achieving this goal, we have identified a novel heptameric peptide (YTYQGKL) that functions as a localization moiety to mediate gene transfer in murine DCs. The sequence was identified by screening a phage display library against a DC cell line (JAWSII) using mouse TNFalpha as the eluting ligand. Alignment analysis reveals YTYQGKL resembles a solvent accessible region in mouse and human TNFalpha structures. A cyclized synthetic peptide bearing the sequence CYTYQGKLC binds to DCs in a concentration-dependent manner. Appending the cyclic peptide to a DNA binding domain (16 consecutive lysine residues) enhances transfection of reporter gene-encoding plasmids in JAWSII cells and in bone marrow derived primary DCs (BMDC). Further enhancement of gene transfer was observed when the peptide-DNA construct was anchored onto polymeric microspheres, with up to 25% of BMDC expressing the transgene. Exposing cells to the free peptide prior to transfection significantly diminished transgene expression. These results demonstrate that YTYQGKL can be used to facilitate ge...Continue Reading

Citations

Dec 5, 2013·Chemical Reviews·Bethany Powell Gray, Kathlynn C Brown
Jul 25, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maliha Zahid, Paul D Robbins
Jul 30, 2005·Expert Opinion on Biological Therapy·Wilson S Meng, Lisa H Butterfield
Oct 27, 2004·Expert Opinion on Biological Therapy·Joseph G AltinChristopher R Parish
Jul 15, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Thomas P Herringson, Joseph G Altin
Feb 3, 2009·Journal of Immunological Methods·Matthias BrosAngelika B Reske-Kunz
Dec 4, 2010·International Journal of Cancer. Journal International Du Cancer·Abdus Faham, Joseph G Altin
May 29, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Maritsa MargaroniEvdokia Karagouni
Feb 26, 2016·Biotechnology and Bioengineering·Michael SkoumalLonnie Shea
Feb 26, 2016·International Journal of Molecular Sciences·Ana DincaMichael T Chin
Jul 14, 2007·Journal of Leukocyte Biology·Abdolamir LandiSylvia van Drunen Littel-van den Hurk
Nov 1, 2016·Biomedit︠s︡inskai︠a︡ khimii︠a︡·G A Kuzmicheva, V A Belyavskaya
Mar 7, 2020·Pharmaceutics·Rebecca E Taylor, Maliha Zahid
Dec 22, 2017·Journal of Cellular and Molecular Medicine·Tal Erlich-HadadHaya Lorberboum-Galski
Mar 10, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hong XuChuanbin Mao
Nov 10, 2005·Chemical Reviews·John W Kehoe, Brian K Kay
Jul 3, 2021·Pharmaceutics·Jeffrey StiltnerMaliha Zahid

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