Gene delivery to muscle

Current Protocols in Human Genetics
Matthew L SpringerHelen M Blau

Abstract

The delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or "knockout" mouse models. This unit describes the isolation and retroviral transduction of mouse myoblasts, the injection of myoblasts and plasmid DNA into mouse muscle, and histological methods for analyzing the recipient muscle. A procedure describing the injection of plasmid DNA into muscle with or without electric charge is also included.

References

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Citations

May 13, 2017·Human Molecular Genetics·Madhurima SahaPeter B Kang
Nov 19, 2014·Molecular and Cellular Biology·Adam C MuellerAnindya Dutta
Jul 13, 2014·Stem Cells Translational Medicine·Kai ZouMarni D Boppart
Oct 19, 2011·Molecular and Cellular Biology·Amar J MajmundarM Celeste Simon
Oct 26, 2018·Frontiers in Physiology·Marta K LewandowskaAnna Rostedt Punga
Mar 5, 2015·Journal of Cellular Physiology·Nunciada SalmaDavid E Fisher
Jul 15, 2015·Development·Amar J MajmundarM Celeste Simon

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