Gene discovery in the adenophorean nematode Trichinella spiralis: an analysis of transcription from three life cycle stages

Molecular and Biochemical Parasitology
Makedonka MitrevaJames P McCarter

Abstract

Expressed sequence tags (ESTs) were produced from cDNA libraries for immature L1, mature muscle larva and adult stages of the adenophorean nematode Trichinella spiralis. 10,130 ESTs were grouped into 3454 gene clusters. The clusters represent a conservative estimate of 3262 unique genes. Interspecific comparisons of the predicted proteins support an ancient divergence of clade I nematodes from other nematodes in the phylum Nematoda. Furthermore, apparent clade I or Trichocephalida-specific proteins were identified, which may include molecular determinants important in the evolution of these species. Similarity matches identified 463 C. elegans genes homologs that confer phenotypes by RNA interference. Classification of predicted proteins suggested diverse cellular, metabolic and extracellular functions, significantly expanding the dataset of T. spiralis proteins with prospective, and potentially critical, functions. Several lines of evidence suggested stage-specific expression of certain genes beyond those previously identified. Evidence was obtained for the existence of large gene families encoding isoforms of known secreted proteins, such as p43 and TspE1. Unexpectedly, diverse isoforms of the muscle larva p43 gene appear to ...Continue Reading

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Citations

Nov 7, 2008·Parasitology Research·Isao NaganoYuzo Takahashi
Apr 11, 2013·Molecular Genetics and Genomics : MGG·Zhengyuan WangMakedonka Mitreva
Jul 3, 2013·International Journal for Parasitology·Edoardo Pozio, Dante S Zarlenga
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Apr 28, 2005·BMC Genomics·Makedonka MitrevaRichard K Wilson
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Apr 7, 2009·Veterinary Parasitology·Sahar AbubuckerMakedonka Mitreva
Jun 15, 2007·Veterinary Parasitology·Makedonka MitrevaDouglas P Jasmer
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