Oct 24, 2018

Gene Disruption Using CRISPR-Cas9 Technology

Methods in Molecular Biology
Nan Hu, Sami N Malek

Abstract

The emergence of the clustered, regularly interspaced, short palindromic repeat (CRISPR) technology provides tools for researchers to modify genomes in a specific and efficient manner. The Type II CRISPR-Cas9 system enables gene editing by directed DNA cleavage followed by either non-homologous end joining (NHEJ) or homology-directed repair (HDR). Here, we described the use of the Type II CRISPR-Cas9 system in detail from designing the guides to analyzing the desired gene disruption events.

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Mentioned in this Paper

Non-Homologous DNA End-Joining
Genome
Genes
CRISPR-Cas Systems
Gene Editing
GATA3
Graft-vs-Host Disease
Research Personnel
NHEJ1
DNA Cleavage

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