Gene duplication and co-evolution of G1/S transcription factor specificity in fungi are essential for optimizing cell fitness

PLoS Genetics
Adi HendlerAmir Aharoni

Abstract

Transcriptional regulatory networks play a central role in optimizing cell survival. How DNA binding domains and cis-regulatory DNA binding sequences have co-evolved to allow the expansion of transcriptional networks and how this contributes to cellular fitness remains unclear. Here we experimentally explore how the complex G1/S transcriptional network evolved in the budding yeast Saccharomyces cerevisiae by examining different chimeric transcription factor (TF) complexes. Over 200 G1/S genes are regulated by either one of the two TF complexes, SBF and MBF, which bind to specific DNA binding sequences, SCB and MCB, respectively. The difference in size and complexity of the G1/S transcriptional network across yeast species makes it well suited to investigate how TF paralogs (SBF and MBF) and DNA binding sequences (SCB and MCB) co-evolved after gene duplication to rewire and expand the network of G1/S target genes. Our data suggests that whilst SBF is the likely ancestral regulatory complex, the ancestral DNA binding element is more MCB-like. G1/S network expansion took place by both cis- and trans- co-evolutionary changes in closely related but distinct regulatory sequences. Replacement of the endogenous SBF DNA-binding domain (...Continue Reading

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Citations

Jul 27, 2017·Current Genetics·Adi HendlerAmir Aharoni
Oct 11, 2019·Current Opinion in Genetics & Development·Edgar M MedinaNicolas E Buchler
Jun 2, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Gabriel S MatosMonica Montero-Lomelí

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Methods Mentioned

BETA
light microscopy
RNA-seq
PCR
ImmunoPrecipitation
ChIP
RNA seq

Software Mentioned

MEME
FIMO
INS
STAR
R
Ensambl
RAxML
MAFFT
ZORRO
FastQC

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