Gene expression alterations by conditional knockout of androgen receptor in adult sertoli cells of Utp14b(jsd/jsd) (jsd) mice.

Biology of Reproduction
Wei ZhouMarvin L Meistrich

Abstract

Spermatogenesis is dependent primarily on testosterone action on the Sertoli cells, but the molecular mechanisms have not been identified. Attempts to identify testosterone-regulated target genes in Sertoli cells have used microarray analysis of gene expression in mice lacking the androgen receptor (AR) in Sertoli cells (SCARKO) and wild-type mice, but the analyses have been complicated both by alteration of germ cell composition of the testis when pubertal or adult mice were used and by differences in Sertoli-cell gene expression from the expression in adults when prepubertal mice were used. To overcome these limitations and identify AR-regulated genes in adult Sertoli cells, we compared gene expression in adult jsd (Utp14b(jsd/jsd), juvenile spermatogonial depletion) mouse testes and with that in SCARKO-jsd mouse testes, since their cellular compositions are essentially identical, consisting of only type A spermatogonia and somatic cells. Microarray analysis identified 157 genes as downregulated and 197 genes as upregulated in the SCARKO-jsd mice compared to jsd mice. Some of the AR-regulated genes identified in the previous studies, including Rhox5, Drd4, and Fhod3, were also AR regulated in the jsd testes, but others, such ...Continue Reading

References

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Citations

Oct 18, 2011·Asian Journal of Andrology·Qiao-Xia ZhangChawnshang Chang
Oct 8, 2020·Molecular Biology of the Cell·Hailey LaroseMary Ann Handel

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Datasets Mentioned

BETA
GSE20918

Methods Mentioned

BETA
electrophoresis
PCR
Fluorescence

Software Mentioned

PATSER
BioConductor
Significance Analysis of Microarrays
Excel
Stereo Investigator
Affy package
Gene
Rotor

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