Gene expression alterations in activated human T-cells induced by modeled microgravity

Journal of Cellular Biochemistry
Nancy E WardD Risin

Abstract

Studies conducted in real Space and in ground-based microgravity analog systems (MAS) have demonstrated changes in numerous lymphocyte functions. In this investigation we explored whether the observed functional changes in lymphocytes in MAS are associated with changes in gene expression. NASA-developed Rotating Wall Vessel (RWV) bioreactor was utilized as a MAS. Activated T lymphocytes were obtained by adding 100 ng/ml of anti-CD3 and 100 U/ml of IL-2 in RPMI medium to blood donor mononuclear cells for 4 days. After that the cells were washed and additionally cultured for up to 2 weeks with media (RPMI, 10% FBS and 100 U/ml IL-2) replacement every 3-4 days. Flow cytometry analysis had proven that activated T lymphocytes were the only cells remaining in culture by that time. They were split into two portions, cultured for additional 24 h in either static or simulated microgravity conditions, and used for RNA extraction. The gene expression was assessed by Affymetrix GeneChip Human U133A array allowing screening for expression of 18,400 genes. About 4-8% of tested genes responded to MG by more than a 1.5-fold change in expression; however, reproducible changes were observed only in 89 genes. Ten of these genes were upregulated a...Continue Reading

References

Jun 1, 1977·Biochemical Genetics·G A Bruns, V M Regina
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T TakizawaA Yoshida
Jul 13, 1984·Science·A CogoliP Fuchs-Bislin
Sep 1, 1993·Journal of Leukocyte Biology·A Cogoli
Jan 1, 1993·Vaccine·A Cogoli
May 1, 1993·Journal of Leukocyte Biology·A CogoliO Müller
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Feb 24, 1997·Biochemical and Biophysical Research Communications·T McNallyD N Halbert
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·N R PellisG F Spaulding
Jan 24, 1998·Human Genetics·W Albig, D Doenecke
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·D H KaplanR D Schreiber
Jul 1, 1998·Clinical Immunology and Immunopathology·J A Hedrick, A Zlotnik
Aug 13, 1998·Nature Medicine·B R Unsworth, P I Lelkes
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·D DiSepioS Nagpal
Dec 19, 1998·The EMBO Journal·A Ciechanover
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G ZhangJ Hurwitz
Jun 22, 1999·Molecular and Cellular Biology·C Suñé, M A Garcia-Blanco
Jul 17, 1999·Journal of Inherited Metabolic Disease·L K Medina-KauweK M Gibson
Nov 11, 1999·Nucleic Acids Research·T A GrayR D Nicholls
Dec 2, 1999·Biochimica Et Biophysica Acta·E MiyoshiN Taniguchi
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C MoratzJ H Kehrl
Apr 7, 2000·Journal of Biotechnology·N L van HalJ Keijer
May 29, 2000·The Journal of Biological Chemistry·J N SpelbrinkH T Jacobs
Jun 14, 2000·Current Opinion in Microbiology·C A HarringtonJ Retief
Jul 14, 2000·The Journal of Biological Chemistry·R T RichardsonM G O'Rand
Aug 29, 2000·Immunopharmacology·P Moseley
May 3, 2001·In Vitro Cellular & Developmental Biology. Animal·D CooperN R Pellis

❮ Previous
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Citations

Jul 22, 2011·The Journal of Biological Chemistry·Lingegowda S MangalaHonglu Wu
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Claudia UlbrichDaniela Grimm
Jan 28, 2014·Brain, Behavior, and Immunity·Brian CrucianClarence Sams
Oct 11, 2015·CNS Neuroscience & Therapeutics·Li-Li ZhangYi-Ling Cai
Nov 15, 2012·Journal of Cellular Biochemistry·Mamta Patel Nagaraja, Diana Risin
Apr 25, 2012·Journal of Neuroscience Methods·Ming-Chang ChiangKuan-Hung Lin
Apr 14, 2018·Radiation Research·Afshin BeheshtiSylvain V Costes
May 28, 2010·Journal of Cellular Biochemistry·Kamaleshwar P SinghJames W Dumond
May 6, 2009·Journal of Cellular Biochemistry·Ragini KumariJames W Dumond
Feb 27, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ye ZhangHonglu Wu
Nov 25, 2018·International Journal of Molecular Sciences·Maria Moreno-VillanuevaHonglu Wu
Jul 14, 2017·Scientific Reports·Cora S ThielOliver Ullrich
Feb 6, 2021·Life·Macauley J GreenPhilip M Williams
Apr 15, 2021·Journal of Biological Engineering·Oscar Fabian Garcia-AponteBence Kozma
Dec 17, 2021·Frontiers in Cell and Developmental Biology·Aaron H Rosenstein, Virginia K Walker

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