Gene expression analysis in hypoplastic lungs in the nitrofen model of congenital diaphragmatic hernia

Journal of Pediatric Surgery
Carmen Mesas BurgosMagnus Nord

Abstract

Pulmonary hypoplasia and persistent pulmonary hypertension are the main causes of mortality and morbidity in newborns with congenital diaphragmatic hernia (CDH). Nitrofen is well known to induce CDH and lung hypoplasia in a rat model, but the mechanism remains unknown. To increase the understanding of the underlying pathogenesis of CDH, we performed a global gene expression analysis using microarray technology. Pregnant rats were given 100 mg nitrofen on gestational day 9.5 to create CDH. On day 21, fetuses after nitrofen administration and control fetuses were removed; and lungs were harvested. Global gene expression analysis was performed using Affymetrix Platform and the RAE 230 set arrays. For validation of microarray data, we performed real-time polymerase chain reaction and Western blot analysis. Significantly decreased genes after nitrofen administration included several growth factors and growth factors receptors involved in lung development, transcription factors, water and ion channels, and genes involved in angiogenesis and extracellular matrix. These results could be confirmed with real-time polymerase chain reaction and protein expression studies. The pathogenesis of lung hypoplasia and CDH in the nitrofen model in...Continue Reading

References

Sep 1, 1969·The Journal of Physiology·T M AdamsonL B Strang
Dec 1, 1995·American Journal of Respiratory Cell and Molecular Biology·A J NorthP W Shaul
Nov 3, 1998·Pediatric Surgery International·D MajorR Cloutier
Jan 28, 2003·The American Journal of Pathology·Jörg MeyJohn J Greer
Mar 7, 2003·Pediatric Research·John J GreerBernard Thebaud
Jun 9, 2004·Journal of Pediatric Surgery·Robert ChangPatricia A D'Amore
Aug 31, 2004·Drug News & Perspectives·Christian WaeberSalvatore Salomone
Feb 9, 2005·Paediatric Respiratory Reviews·Alison Hislop
Feb 16, 2005·Annual Review of Physiology·Kurt R Stenmark, Steven H Abman
Mar 29, 2005·Journal of Pediatric Surgery·Björn FrencknerLena Sahlin
Apr 9, 2005·Pediatric Research·David WarburtonWei Shi
Jul 30, 2005·Seminars in Perinatology·Fernando R Moya, Kevin P Lally
Jul 30, 2005·Seminars in Perinatology·Sonlee D West, Jay M Wilson
Dec 16, 2005·Fetal Diagnosis and Therapy·F BargyP Barbet
Aug 16, 2006·European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [et Al] = Zeitschrift Für Kinderchirurgie·C Mesas-BurgosB Frenckner
Jan 5, 2007·Pediatric Surgery International·Nana NakazawaPrem Puri
Feb 3, 2007·Journal of Pediatric Surgery·Nana NakazawaPrem Puri
Feb 3, 2007·Journal of Pediatric Surgery·Hajime TakayasuPrem Puri
Feb 17, 2007·American Journal of Respiratory and Critical Care Medicine·Olivier BoucheratJacques R Bourbon
Apr 28, 2007·Seminars in Pediatric Surgery·Robin D Clugston, John J Greer
Aug 21, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·B Rhiannon NobleJohn J Greer
Feb 19, 2008·Journal of Pediatric Surgery·Hajime TakayasuPrem Puri
Aug 1, 2008·Pediatric Surgery International·Andreas RingmanBjörn Frenckner
Apr 14, 2009·Journal of Pediatric Surgery·Carmen Mesas-BurgosBjörn Frenckner
Aug 5, 2009·Pediatric Surgery International·Andreas Ringman UgglaBjörn Frenckner

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Citations

May 10, 2011·Revue des maladies respiratoires·A LabbéP-J Déchelotte
Feb 14, 2012·Pediatric Pulmonology·Aline VuckovicJacques C Jani
Nov 29, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·G Ulrich-MerzenichH Wagner
Sep 28, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jose Luis PeiroBrian M Varisco

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