Gene expression analysis of chromosomal regions with gain or loss of genetic material detected by comparative genomic hybridization

Genes, Chromosomes & Cancer
Bárbara MeléndezJ Benítez

Abstract

Comparative genomic hybridization (CGH) has been widely used to detect copy number alterations in cancer and to identify regions containing candidate tumor-responsible genes; however, gene expression changes have been described only in highly amplified regions (amplicons). To study the overall impact of slight copy number changes on gene expression, we analyzed 16 T-cell lymphomas by using CGH and a custom-designed cDNA microarray containing 7,657 genes and expressed sequence tags related to tumorigenesis. We evaluated mean gene expression and variability within CGH-altered regions and explored the relationship between the effects of the gene and its position within these regions. Minimally overlapping CGH candidate areas (6q25, 13q21-q22, and 19q13.1) revealed a weak relationship between altered genomic content and gene expression. However, some candidate genes showed modified expression within these regions in the majority of tumors; these candidate genes were evaluated and confirmed in another independent series of 23 T-cell lymphomas by use of the same cDNA microarray and by FISH on a tissue microarray. When all the CGH regions detected for each tumor were considered, we found a significant increase or decrease in the mean ...Continue Reading

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Citations

Mar 11, 2005·Current Opinion in Obstetrics & Gynecology·Jean-Michel Lapierre, Gérard Tachdjian
Feb 24, 2009·Critical Reviews in Oncology/hematology·Laurence de LevalPhilippe Gaulard
Oct 22, 2005·Mental Retardation and Developmental Disabilities Research Reviews·Rong Mao, Jonathan Pevsner
Aug 19, 2008·International Journal of Cancer. Journal International Du Cancer·Alexander MarméHerwig Ponstingl

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