Gene expression analysis of human endometrial endothelial cells exposed to op'-DDT

Molecular Human Reproduction
Carolina BredhultMatts Olovsson

Abstract

The endocrine disrupting chemical o, p'-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 microM o,p'-DDT decreased their proliferation compared with the control. Microarray analyses revealed that o,p'-DDT affected biological processes such as the cell cycle, cell division, defence response and lipid and steroid metabolism, in cellular components such as the plasma membrane and chromosomes, with molecular functions involved in signalling, receptor and cytokine activity, confirming the results of the proliferation assay. Expression of five of the most differentially expressed genes identified in the microarray analysis was verified by real-time quantitative reverse transcription polymerase chain reaction in five HEEC cultures obtained from women in the proliferative phase and in five cultures obtained from women in the secretory phase of the menstrual cycle after treatment with o,p'-DDT. The present study supports our previous findings of decreased proliferation and increased cell death in response to o,p'-DDT and may offer important clues to the mechanisms of action of o,p'-DDT.

References

Apr 1, 1987·Archives of Toxicology·S P SaxenaJ Chandra
Sep 1, 1973·Comparative and General Pharmacology·D E PeakallW B Kinter
Jan 1, 1971·Journal of Agricultural and Food Chemistry·H C CecilS J Harris
Mar 1, 1969·Toxicology and Applied Pharmacology·R M WelchA H Conney
Jul 1, 1984·Fertility and Sterility·M TrappW Heeschen
May 1, 1995·Environmental Health Perspectives·L J GuilletteH F Percival
Mar 7, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·C D Lundholm
Feb 18, 1999·Toxicology Letters·C BergI Brandt
Mar 27, 1999·Archives of Environmental Health·L Torres-ArreolaM López-Cervantes
May 13, 1999·The Journal of Biological Chemistry·K HirataT Quertermous
Feb 12, 2002·Bioinformatics·Alexander SturnZlatko Trajanoski
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 5, 2003·Lancet·Barbara A CohnPentti K Siiteri
Nov 25, 2003·The EMBO Journal·Luisa Di StefanoKristian Helin
Apr 6, 2004·Environmental Health Perspectives·Félix Salazar-GarcíaVictor H Borja-Aburto
Nov 10, 2004·Environmental Research·Matthew P LongneckerJohn W Brock
Dec 23, 2004·The Journal of Clinical Endocrinology and Metabolism·Graciela KrikunCharles J Lockwood
Aug 4, 2005·Endocrine·Alison C HollowayWarren G Foster
Nov 16, 2005·Hormone Research·Anne-Simone ParentJean-Pierre Bourguignon
Feb 1, 2006·Archives of Environmental Contamination and Toxicology·Nandong Xue, Xiaobai Xu
Feb 4, 2006·Bioinformatics·Adam AmeurJan Komorowski
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
May 12, 2006·Biology of Reproduction·Belgin SelamAydin Arici
May 30, 2006·Environmental Research·Celeste Matos Lino, M Irene Noronha da Silveira
Jun 7, 2006·Epidemiology·Gunnar ToftJens P Bonde

❮ Previous
Next ❯

Citations

Jul 26, 2013·Reproductive Sciences·Malin HelmestamMatts Olovsson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.