Gene-expression analysis of polyI:C-stimulated primary human conjunctival epithelial cells

The British Journal of Ophthalmology
Mayumi UetaShigeru Kinoshita

Abstract

The authors previously reported that human ocular surface epithelium expressed TLR3 and that its ligand polyI:C stimulated the secretion of IL-6, IL-8 and IFN-β. In this study, to examine comprehensive effects of polyI:C stimulation of primary human conjunctival epithelial cells (PHCjECs), the authors performed a gene-expression analysis of the polyI:C-stimulated PHCjECs using oligonucleotide microarrays, GeneChip. The transcripts upregulated upon polyI:C stimulation in PHCjECs from two individuals were examined using GeneChip. Eleven new upregulated transcripts of interest were confirmed by quantitative real-time PCR (RT-PCR), and seven proteins produced by those transcripts were examined by ELISA or immunoblot analysis in PHCjECs from three other individuals, respectively. GeneChip analysis showed that 150 transcripts were upregulated more than threefold and that 47 transcripts were upregulated more than 10-fold upon polyI:C stimulation in the PHCjECs. Eleven of the 47 upregulated transcripts (CXCL11, RIG-I, IL28A, CXCL10, CCL5, CCL4, MDA5, IL7R, TSLP, CCL20 and ICAM-1) were significantly upregulated upon polyI:C stimulation by quantitative RT-PCR, and the levels of seven proteins of the transcripts CXCL11, CXCL10, CCL5, CCL2...Continue Reading

Citations

May 28, 2011·Japanese Journal of Ophthalmology·Mayumi UetaShigeru Kinoshita
May 15, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Mayumi UetaShigeru Kinoshita
Apr 17, 2014·PloS One·Radhika SusarlaSaaeha Rauz
Sep 17, 2013·Immunology Letters·Mayumi UetaShigeru Kinoshita
Jun 26, 2012·Progress in Retinal and Eye Research·Mayumi Ueta, Shigeru Kinoshita
Jan 29, 2011·Clinical Science·Alessandro LambiaseStefano Bonini
Jan 4, 2013·Current Eye Research·Daniel R SabanJerry Y Niederkorn
Nov 28, 2018·Investigative Ophthalmology & Visual Science·Mayumi Ueta
Feb 12, 2020·Expert Review of Clinical Immunology·Mayumi Ueta

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