Gene expression analysis of purified hematopoietic stem cells and committed progenitors.

Blood
Alexey V TerskikhIrving L Weissman

Abstract

Lifelong self-renewal is a unique property of somatic stem cells. Recently, several primitive multipotent yet committed (non-self-renewing) hematopoietic progenitor populations were identified in mouse bone marrow. We have characterized the expression of 1200 selected mouse genes using the Atlas cDNA array in highly purified hematopoietic stem cells (HSCs) and 6 closely related progenitor populations: common myeloid progenitors (CMPs), granulocyte-macrophage progenitors (GMPs), megakaryocyte-erythrocyte progenitors (MEPs), common lymphoid progenitors (CLPs), and pro-T and pro-B cells. Cluster analysis revealed that nearly half of all differentially expressed transcripts are associated with HSCs, supporting the notion of an active transcriptional status of HSCs. Genes found enriched in the HSC cluster encompass many developmentally regulated genes, some previously associated with HSC self-renewal. In contrast, genes that are enriched in committed progenitors are mostly associated with hematopoietic differentiation, immune regulation, and metabolism. Thus, the transition from HSCs toward committed progenitors correlates with the down-regulation of a large number of HSC-associated genes and progressive up-regulation of a limited n...Continue Reading

References

May 1, 1979·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·R O MorganA Barbeau
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Aug 1, 1995·Bioscience, Biotechnology, and Biochemistry·S Takamatsu, K Nakano
Nov 1, 1995·Molecular and Cellular Biology·C McCoyM M Cornwell
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T SudoS Nishikawa
Aug 1, 1997·Journal of Leukocyte Biology·J A Hamilton
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·S KimuraW S Alexander
Feb 26, 2000·Science·F M Watt, B L Hogan
Feb 26, 2000·Science·F H Gage
Jun 2, 2000·Science·R L PhillipsI R Lemischka
Nov 9, 2000·The Journal of Experimental Medicine·H EmaH Nakauchi
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·A V TerskikhI L Weissman
Sep 15, 2001·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·V LászlóA Falus
Nov 2, 2001·Nature·T ReyaI L Weissman
Jul 12, 2002·Developmental Cell·Toshihiro MiyamotoKoichi Akashi

❮ Previous
Next ❯

Citations

Nov 19, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Zsuzsanna HorváthEdit I Buzás
Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Vincent E SollarsArthur M Buchberg
Oct 26, 2007·Cell and Tissue Research·Stefan Zimmermann, Uwe M Martens
Mar 25, 2010·Clinical & Experimental Metastasis·Lei XuRichard O Hynes
Dec 5, 2006·Stem Cell Reviews·E Camilla ForsbergIrving L Weissman
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Ilaria Bellantuono
Nov 30, 2002·Seminars in Immunology·Susan S ProhaskaMotonari Kondo
Oct 25, 2007·Nature Reviews. Cancer·Andrei V Krivtsov, Scott A Armstrong
Aug 17, 2005·Nature Reviews. Molecular Cell Biology·Craig E EckfeldtCatherine M Verfaillie
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Emmanuelle PasseguéIrving L Weissman
Aug 2, 2006·The Journal of Experimental Medicine·Anne Y Lai, Motonari Kondo
May 14, 2008·The Journal of Experimental Medicine·Benjamin J ThompsonIannis Aifantis
May 4, 2010·Cellular Reprogramming·Ming-Tao ZhaoRandall S Prather
Apr 18, 2006·Critical Care Medicine·Brian R Curtis, Janice G McFarland
Jul 17, 2004·Current Opinion in Hematology·Kateri A Moore
Jun 7, 2006·Current Opinion in Hematology·Paul A Ney
Mar 23, 2006·Annual Review of Immunology·James P Di Santo
Sep 3, 2005·Oncology·József TímárKároly Lapis
Feb 25, 2006·The Journal of Clinical Investigation·Hui JinJudy Varner
May 12, 2010·The Journal of Clinical Investigation·Alexey BersenevWei Tong
Sep 10, 2005·PLoS Genetics·E Camilla ForsbergIrving L Weissman
Sep 15, 2005·International Journal of Hematology·Toshihiro Miyamoto, Koichi Akashi
Oct 20, 2006·International Journal of Hematology·Katsutoshi OzakiTetsuya Nosaka
May 8, 2009·International Journal of Medical Sciences·Sveta KabanovaThomas W Jax
Aug 11, 2005·PLoS Biology·Craig E EckfeldtCatherine M Verfaillie
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Chance John LuckeyDiane Mathis
Jun 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lei XuRichard O Hynes
Sep 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Regis DoyonnasHelen M Blau
Dec 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhi-jia YeSherman M Weissman
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Chia-Lin HsuMotonari Kondo
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Vladimir JankovicStephen D Nimer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Related Papers

Science
Natalia B IvanovaIhor R Lemischka
Proceedings of the National Academy of Sciences of the United States of America
J L Christensen, Irving L Weissman
Archives of Biochemistry and Biophysics
P A Wiersma, G S Cox
© 2021 Meta ULC. All rights reserved