Gene expression analysis of Salmonella enterica Enteritidis Nal(R) and Salmonella enterica Kentucky 3795 exposed to HCl and acetic acid in rich medium.

Foodborne Pathogens and Disease
Rolf D JoergerSteffen Porwollik

Abstract

In the United States, serovar Kentucky has become one of the most frequently isolated Salmonella enterica serovars from chickens. The reasons for this prevalence are not well understood. Phenotypic comparisons of poultry Salmonella isolates belonging to various serovars demonstrated that serovar Kentucky isolates differed from those of most other serovars in their response to acid. Microarray and qPCR analyses were performed with aerated exponentially growing poultry isolates, Salmonella enterica serovar Kentucky 3795 and Enteritidis Nal(R), exposed for 10 min to tryptic soy broth (TSB) adjusted to pH 4.5 with HCl and to pH 5.5 with HCl or acetic acid. Data obtained by microarray analysis indicated that more genes were up- or down-regulated in strain Kentucky 3795 than in Enteritidis Nal(R) under acidic conditions. Acid exposure in general caused up-regulation of energy metabolism genes and down-regulation of protein synthesis genes, particularly of ribosomal protein genes. Both strains appear to similarly utilize the lysine-based pH homeostasis system, as up-regulation of cadB was observed under the acidic conditions. Expression of regulatory genes (rpoS, fur, phoPQ) known to be involved in the acid response showed similar tre...Continue Reading

References

Aug 1, 1991·Journal of Bacteriology·J W Foster, H K Hall
Feb 1, 1990·Journal of Bacteriology·J W Foster, H K Hall
Jan 1, 1995·Critical Reviews in Microbiology·J W Foster
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A T MaurelliA Fasano
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R HoskinsS Wickner
Mar 27, 1999·Journal of Bacteriology·E SuziedelienéS Normark
Oct 16, 2001·Mutation Research·S PorwollikM McClelland
May 25, 2002·Journal of Bacteriology·David G KehresMichael E Maguire
Mar 26, 2003·Nucleic Acids Research·Steffen PorwollikMichael McClelland
Aug 18, 2004·Research in Microbiology·Vaida SeputieneEdita Suziedeliene
Dec 6, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masami UetaAkira Wada
Dec 24, 2005·BMC Bioinformatics·Xiaoqin XiaYipeng Wang
Jul 21, 2006·Journal of Bacteriology·Jasper Kieboom, Tjakko Abee
Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thomas HenryStéphane Méresse
Nov 7, 2006·Journal of Bacteriology·Renée KernGilbert Richarme
May 9, 2007·Nucleic Acids Research·Andreas UntergasserJack A M Leunissen
Sep 5, 2007·Microbiology·Martín EsparizFernando C Soncini
Sep 17, 2008·Infection and Immunity·Roberto C Vazquez-JuarezAlfredo G Torres
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lee Macomber, James A Imlay
Jul 16, 2009·Bioinformatics·Xiao-Qin XiaYipeng Wang

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Citations

Feb 7, 2015·Foodborne Pathogens and Disease·Rolf D Joerger, Steven Choi
Dec 4, 2013·Microbiology and Molecular Biology Reviews : MMBR·Steven L FoleyJessica Danzeisen
Feb 8, 2019·Journal of Food Science·Beining YeXianming Shi

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Datasets Mentioned

BETA
GSE26597

Methods Mentioned

BETA
PCR
protein folding

Software Mentioned

PrimerPlus
WebArrayDB
GenePix Pro
QuantArray
ScanArray Express
JMP

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