Gene expression and antimicrobial activity of bovine macrophages in response to Mycobacterium avium subsp. paratuberculosis

Veterinary Pathology
D J WeissMitchell S Abrahamsen

Abstract

We evaluated gene expression and antimicrobial responses of bovine monocyte-derived macrophages incubated with Mycobacterium avium subsp. paratuberculosis (M. a. ptb), the causative agent of Johne's disease. Gene expression was evaluated by the use of human noncompetitive high-density oligonucleotide microarrays. Bovine messenger RNA hybridized with 14.2-18.2% of the 12,600 oligonucleotide probe sets. When macrophages incubated with M. a. ptb were compared with nonactivated control macrophages, macrophages activated by addition of interferon-gamma and lipopolysaccharide, and macrophages incubated with Mycobacterium avium subspecies avium (M. a. a), 47, 79, and 27 genes, respectively, were differentially expressed. Differential expression of six of these genes was confirmed using reverse transcriptase polymerase chain reaction. Several functional assays were performed to evaluate the potential relevance of differentially expressed genes to host defense. Macrophages phagocytizing M. a. a had a greater capacity to kill the organisms and to acidify phagosomes and a greater degree of apoptosis than did macrophages incubated with M. a. ptb. The results of these studies indicate that multiple genes and metabolic pathways are different...Continue Reading

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Citations

Sep 5, 2006·BMC Genomics·Kirsty JensenElizabeth J Glass
Dec 30, 2009·Infection and Immunity·Gabriella M ScandurraDesmond M Collins
Apr 6, 2005·Veterinary Immunology and Immunopathology·Heather L WilsonPhilip J Griebel
Apr 6, 2005·Veterinary Immunology and Immunopathology·Kirsty McGuire, Elizabeth J Glass
Jul 20, 2006·Apoptosis : an International Journal on Programmed Cell Death·Daniel P Barry, Blaine L Beaman
Sep 6, 2005·Veterinary Immunology and Immunopathology·Bryce M BuddleMargot A Skinner
Feb 26, 2008·Journal of Veterinary Science·Seng Ryong Woo, Charles J Czuprynski
Sep 19, 2019·Infection and Immunity·Kathryn WrightKumudika de Silva
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Palazzo FiorentinaGiulietta Minozzi

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
in vitro transcription
PCR
light microscopy
chips
chip

Software Mentioned

Affymetrix Microarray Suite
Image
Pro Plus
Affymetrix

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis