Gene expression and developmental competence of bovine embryos produced in vitro with different serum concentrations

Reproduction in Domestic Animals = Zuchthygiene
A T D OliveiraJ L Rodrigues

Abstract

The objective of this study was to determine the developmental rates and relative abundance of Hsp 70.1 and Glut-1 transcripts in in vivo- and in vitro-produced (IVP) bovine embryos in media supplemented with bovine serum albumin (BSA) or different oestrous cow serum concentrations. In experiment 1, in vitro maturation and culture media were supplemented with 0.4% BSA or 1, 5, 10 or 20% of oestrous cow serum (ECS). The analysis of the expression of Hsp 70.1 and Glut-1 was carried out in individual days 7 and 8 embryos by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay. In experiment 2, in vivo-produced morulae were collected on day 7 of the oestrous cycle and employed for the comparison of the relative abundances of Hsp 70.1 and Glut-1 transcripts with IVP morulae produced using two protein sources (10% ECS or 0.4% BSA). No differences were observed in cleavage rate among groups, but blastocyst formation (27%) and hatching rates (78%) were significantly higher in IVP embryos produced with 20% ECS than the other groups (p<0.05). No significant differences were observed in the relative abundances of Hsp 70.1 and Glut-1 mRNA in days 7 and 8 blastocysts expanded blastocysts between groups. The abu...Continue Reading

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Citations

May 10, 2005·Theriogenology·Alexandre Tavares Duarte de OliveiraJosé Luiz Rodrigues
Oct 31, 2007·Zygote : the Biology of Gametes and Early Embryos·H BadrR Puglisi
Mar 17, 2009·Molecular Reproduction and Development·M N PurperaK R Bondioli
May 21, 2013·Theriogenology·N N CostaO M Ohashi
Aug 24, 2013·The Journal of Reproduction and Development·Hiroki HirayamaAkira Minamihashi

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