Gene expression and DNA methylation changes in the hypothalamus and hippocampus of adult rats developmentally exposed to bisphenol A or ethinyl estradiol: a CLARITY-BPA consortium study

Epigenetics : Official Journal of the DNA Methylation Society
Ana CheongCheryl S Rosenfeld

Abstract

Bisphenol A (BPA), an endocrine disrupting chemical (EDC), is a ubiquitous pollutant. As part of the Consortium Linking Academic and Regulatory Insights on BPA Toxicity (CLARITY-BPA), we sought to determine whether exposure of Sprague-Dawley rats to 2,500 μg/kg/day BPA (BPA) or 0.5 μg/kg/day ethinyl estradiol (EE) from gestational day 6 through postnatal day 21 induces behavior-relevant gene expression and DNA methylation changes in hippocampus and hypothalamus at adulthood. RNA and DNA were isolated from both regions. Expression of ten genes (Dnmt1, Dnmt3a, Dnmt3b, Esr1, Esr2, Avp, Ar, Oxt, Otr, and Bdnf) presumably altered by early-life BPA/EE exposure was examined. Three genes (Bdnf, Dnmt3b, and Esr1) were studied for DNA methylation changes in their putative 5' promoter regions. Molecular changes in hippocampus were correlated to prior Barnes maze performance, including sniffing correct holes, distance traveled, and velocity. Exposure to BPA and/or EE disrupted patterns of sexually dimorphic gene expression/promoter DNA methylation observed in hippocampus and hypothalamus of controls. In the hippocampus of female offspring, BPA exposure resulted in hypermethylation of the putative 5' promoter region of Bdnf, while EE exposu...Continue Reading

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Citations

Sep 13, 2018·Basic & Clinical Pharmacology & Toxicology·Gail S PrinsLaura N Vandenberg
Jan 18, 2020·Environmental Health Perspectives·Felicia Fei-Lei Chung, Zdenko Herceg
Aug 5, 2020·Journal of Neuroendocrinology·Pascal CoumailleauThierry D Charlier
Mar 8, 2019·Nature Reviews. Endocrinology·Laura N VandenbergAndrea C Gore
May 8, 2019·Journal of Neuroendocrinology·Heather B Patisaul
Aug 1, 2019·Current Neuropharmacology·Antonietta SantoroRosaria Meccariello
Dec 9, 2020·Nature Reviews. Endocrinology·David Lopez-RodriguezAnne-Simone Parent
Jan 29, 2021·Frontiers in Genetics·Tengfei QinXiangFen Xiao
Mar 6, 2021·Environmental Science and Pollution Research International·Nadeem Ghani KhanShama Prasada Kabekkodu
Jul 20, 2020·Reproductive Toxicology·Jerrold J HeindelR Thomas Zoeller

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Methods Mentioned

BETA
RNAseq
transgenic
chip
PCR

Software Mentioned

Primer 3
SAS

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