Gene expression and flavonolignan production in fruits and cell cultures of Silybum marianum

Journal of Plant Physiology
María Torres, Purificación Corchete

Abstract

The hepatoprotectant flavonolignan silymarin (Sm) is synthesized through 4-coumaroyl-CoA, which enters both the flavonoid and the monolignol pathway giving the two immediate precursors taxifolin (Tx) and coniferyl alcohol (CA), respectively. Sm formation occurs via oxidative radicalization of Tx and CA and is accumulated at high levels at final stages of maturation of Silybum marianum fruits. By contrast, Sm production is severely reduced in cell cultures of this species, although suspensions are able to excrete Sm compounds into the medium upon elicitation with methyl jasmonate (MeJA) or cyclodextrins (CD). Knowledge of gene expression is important to understand Sm dynamics and to develop strategies aimed at increasing production by means of cell cultures but, to date, only one gene of the pathway (chalcone synthase, SmCHS) has been cloned. Therefore, to elucidate the relationship between expression of Sm pathway genes and production of these metabolites, four cDNA fragments of genes putatively involved in flavonolignan biosynthesis, chalcone isomerase, flavanone 3-hydroxylase, flavonol 3'-hydroxylase and cinnamyl alcohol dehydrogenase, were isolated from Sm producing S. marianum fruits and their expression, together with that...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 21, 2003·Organic & Biomolecular Chemistry·Nam-Cheol KimMonroe E Wall
Sep 25, 2003·Annual Review of Plant Biology·Wout BoerjanMarie Baucher
Aug 2, 2005·Journal of Biotechnology·M Angeles Sánchez-SampedroPurificación Corchete
Sep 20, 2005·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Vladimír Kren, Daniela Walterová
May 4, 2006·Annual Review of Plant Biology·Loïc LepiniecMichel Caboche
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Laurens PauwelsAlain Goossens
Apr 7, 2011·Applied Microbiology and Biotechnology·Miriana DuranteGiovanni Mita
Sep 8, 2011·Journal of Young Pharmacists : JYP·A GhoshT Ghosh
Sep 13, 2011·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·T T H DaoR Verpoorte
Jul 23, 2013·International Journal of Molecular Sciences·Elisa PetrussaAngelo Vianello
Dec 18, 2013·Journal of Plant Physiology·Daniel Prieto, Purificación Corchete

❮ Previous
Next ❯

Citations

Oct 21, 2016·Plant Physiology and Biochemistry : PPB·Hoda A S El-GarhyFadia El Sherif
Oct 17, 2018·Molecular Biotechnology·Yan-Chao YinYing Liu
Jun 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuxing DengShanfa Lu
Oct 11, 2017·The Plant Journal : for Cell and Molecular Biology·Yongkun LvJian Chen
Aug 8, 2021·International Journal of Molecular Sciences·Vladimír Křen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.