Gene expression and secretion of insulin-like growth factor-II and insulin-like growth factor binding protein-2 from cultured sheep choroid plexus epithelial cells

Brain Research. Molecular Brain Research
N R HolmS Gammeltoft

Abstract

The gene expression of insulin-like growth factor II (IGF-II) and insulin-like growth factor binding protein-2 (IGFBP-2) has previously been demonstrated in rat and human choroid plexus by in situ hybridization analysis. In the present study we have characterized IGF-II and IGFBP-2 transcripts and proteins in primary cultures of epithelial cells from lateral choroid plexus of sheep brain. Northern blot analysis of total RNA showed one major IGF-II mRNA of 4.8 kb and four minor IGF-II transcripts of 1.5, 2.0, 3.0 and 6.0 kb as well as one IGFBP-2 transcript of 1.7 kb. Radioreceptor assay of conditioned medium from the cultured choroid plexus epithelial cells showed inhibition of [125I]IGF-I and [125I]IGF-II binding to mouse NIH 3T3 fibroblasts, the displacement curves being identical to that of unlabelled IGF-II. The conditioned medium was fractionated by gel filtration on a Bio-Gel P-60 column, and analysis by IGF-II radioreceptor assay showed two peaks of IGF-II-binding inhibitory activity of M(r) 7.5-10 and 25 kDa, suggesting the presence of both IGF-II, and an IGFBP. Western immunoblot analysis of conditioned medium with antibodies toward IGF-II and IGFBP-2 demonstrated proteins with M(r) 6 kDa and 32 kDa, respectively. Prot...Continue Reading

References

Jul 5, 1990·European Journal of Biochemistry·R E Humbel
Mar 1, 1989·The Journal of Clinical Endocrinology and Metabolism·R G RosenfeldR C Baxter
Sep 1, 1988·Brain Research·Y IchimiyaR S Gilmour
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F StylianopoulouA Efstratiadis
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G K HaselbacherR E Humbel
Oct 1, 1981·Journal of Neurochemistry·R B CrookS B Prusiner

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Citations

Mar 15, 2012·Cellular and Molecular Life Sciences : CMLS·Mauro W Zappaterra, Maria K Lehtinen
May 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A R AldredG Schreiber
Mar 4, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Ruo L ChenJane E Preston
Mar 1, 1996·The European Journal of Neuroscience·C NilssonS Gammeltoft
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·L LimR H Costa
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Maria K Lehtinen, Christopher A Walsh
Apr 21, 1998·Molecular and Cellular Endocrinology·R C HoveyT B McFadden
Jan 6, 2000·The Journal of Steroid Biochemistry and Molecular Biology·A L QuinnR W Brueggemeier
Nov 2, 1999·Biochemical and Biophysical Research Communications·P M ArnoldB W Festoff
Jan 3, 2001·Microscopy Research and Technique·A Chodobski, J Szmydynger-Chodobska
Jan 3, 2001·Microscopy Research and Technique·J E Preston
May 14, 1998·Microscopy Research and Technique·J B GabrionJ P Delaunoy
May 3, 2007·Journal of Integrative Neuroscience·Justine M GattLeanne M Williams
Feb 24, 2001·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·S OginoM L Cohen
Nov 2, 2019·Journal of Neuroscience Methods·Ulrike ErbMichael Karremann
Nov 17, 2020·Frontiers in Cellular Neuroscience·Arie HorowitzVianney Gilard

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