Gene expression changes and DNA damage after ex vivo exposure of peripheral blood cells to various CT photon spectra.

Scientific Reports
Hanns Leonhard KaatschReinhard Ullmann

Abstract

Dual-energy CT provides enhanced diagnostic power with similar or even reduced radiation dose as compared to single-energy CT. Its principle is based on the distinct physical properties of low and high energetic photons, which, however, may also affect the biological effectiveness and hence the extent of CT-induced cellular damage. Therefore, a comparative analysis of biological effectiveness of dual- and single-energy CT scans with focus on early gene regulation and frequency of radiation-induced DNA double strand breaks (DSBs) was performed. Blood samples from three healthy individuals were irradiated ex vivo with single-energy (80 kV and 150 kV) and dual-energy tube voltages (80 kV/Sn150kV) employing a modern dual source CT scanner resulting in Volume Computed Tomography Dose Index (CTDIvol) of 15.79-18.26 mGy and dose length product (DLP) of 606.7-613.8 mGy*cm. Non-irradiated samples served as a control. Differential gene expression in peripheral blood mononuclear cells was analyzed 6 h after irradiation using whole transcriptome sequencing. DSB frequency was studied by 53BP1 + γH2AX co-immunostaining and microscopic evaluation of their focal accumulation at DSBs. Neither the analysis of gene expression nor DSB frequency pr...Continue Reading

References

Oct 1, 1989·The British Journal of Radiology·D J Brenner, H I Amols
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Nov 14, 2002·International Journal of Radiation Biology·M Frankenberg-SchwagerW Göhde
Apr 30, 2003·Radiation and Environmental Biophysics·Dorota SłoninaWolfgang Dörr
Dec 30, 2004·Radiation Protection Dosimetry·M A Hill
Jun 24, 2008·International Journal of Radiation Oncology, Biology, Physics·Sunirmal Paul, Sally A Amundson
Feb 20, 2009·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Nezahat Hunter, Colin R Muirhead
Jun 1, 2012·European Journal of Radiology·Mitsuru TakeuchiYuta Shibamoto
Nov 30, 2012·Nucleic Acids Research·Tanya BarrettAlexandra Soboleva
Jul 6, 2014·La Radiologia medica·Vitaliano BuffaVittorio Miele
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 17, 2015·Environmental and Molecular Mutagenesis·Kai RothkammSusanne Burdak-Rothkamm
May 23, 2015·BMC Bioinformatics·Henry HeberleRosane Minghim
Oct 27, 2015·AJR. American Journal of Roentgenology·Chiao-Yun ChenGin-Chung Liu
Jan 6, 2016·International Journal of Molecular Sciences·Mykyta Sokolov, Ronald Neumann
Mar 7, 2017·Nature Methods·Rob PatroCarl Kingsford
Mar 14, 2017·Oncogene·M Fischer
Jul 4, 2017·Korean Journal of Radiology : Official Journal of the Korean Radiological Society·Hyun Woo Goo, Jin Mo Goo
Nov 2, 2017·Dento Maxillo Facial Radiology·Juliana Bm da FonteWilton M Takeshita
Sep 14, 2019·International Journal of Molecular and Cellular Medicine·Seyed Masoud JafarpourHossein Safari
Oct 2, 2019·AJR. American Journal of Roentgenology·Geoiphy George PulickalAshish Chawla
Mar 14, 2020·Health Physics·Hanns Leonhard KaatschBenjamin Valentin Becker
Mar 28, 2020·Medical Physics·Cynthia H McColloughNorbert J Pelc

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