Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms

BMC Genomics
Pierre De WitChris J Langdon

Abstract

Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 h post fertilization, and it has been demonstrated that changes in water chemistry can cause delays in shell formation, shell deformations and higher mortality rates. In this study, we use the delay in shell formation associated with exposure to CO2-acidified seawater to identify genes correlated with initial shell deposition. By fitting linear models to gene expression data in ambient and low aragonite saturation treatments, we are able to isolate 37 annotated genes correlated with initial larval shell formation, which can be categorized into 1) ion transporters, 2) shell matrix proteins and 3) protease inhibitors. Clustering of the gene expression data into co-expression networks further supports the result of the linear models, and also implies an important role of dynein motor proteins as transporters of cellular components during the initial shell formation process. Using an RNA-Seq approach with high temporal resolution allows us to identify a conceptual model for how oyster lar...Continue Reading

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Citations

Aug 2, 2020·Biological Reviews of the Cambridge Philosophical Society·Melody S ClarkElizabeth M Harper
Aug 6, 2019·Ecology and Evolution·Kirti RameshFrank Melzner
May 31, 2020·The Journal of Experimental Biology·Melody S Clark
Jan 29, 2020·Ecology and Evolution·Emma Timmins-SchiffmanSteven B Roberts
Feb 26, 2020·Frontiers in Zoology·Marie E StraderGretchen E Hofmann
Mar 20, 2018·Developmental and Comparative Immunology·Zhaoqun LiuLinsheng Song
Dec 7, 2018·Developmental and Comparative Immunology·José A Fernández RobledoMichael J Metzger
Apr 25, 2021·The Science of the Total Environment·Yunlong LiWen-Xiong Wang
May 9, 2021·Global Change Biology·Kanmani Chandra RajanThiyagarajan Vengatesen
Jun 2, 2021·Marine Pollution Bulletin·Said Hamid ThangalThirunavukkarasu Muralisankar
Sep 14, 2021·Frontiers in Physiology·Christopher L Reyes-GilerSarah W Davies

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Methods Mentioned

BETA
protein modification
PCR

Software Mentioned

R
ErmineJ
DESeq
GSR
fastx
WGCNA

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