Gene expression disruptions of organism versus organ in Drosophila species hybrids.

PloS One
Daniel J Catron, Mohamed A F Noor

Abstract

Hybrid dysfunctions, such as sterility, may result in part from disruptions in the regulation of gene expression. Studies of hybrids within the Drosophila simulans clade have reported genes expressed above or below the expression observed in their parent species, and such misexpression is associated with male sterility in multigenerational backcross hybrids. However, these studies often examined whole bodies rather than testes or had limited replication using less-sensitive but global techniques. Here, we use a new RNA isolation technique to re-examine hybrid gene expression disruptions in both testes and whole bodies from single Drosophila males by real-time quantitative RT-PCR. We find two early-spermatogenesis transcripts are underexpressed in hybrid whole-bodies but not in assays of testes alone, while two late-spermatogenesis transcripts seem to be underexpressed in both whole-bodies and testes alone. Although the number of transcripts surveyed is limited, these results provide some support for a previous hypothesis that the spermatogenesis pathway in these sterile hybrids may be disrupted sometime after the expression of the early meiotic arrest genes.

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Citations

Jul 14, 2011·Genetics·John P MaslyMichelle N Arbeitman
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Timothy B SacktonBernardo Lemos
Jun 17, 2010·Molecular Biology and Evolution·Erin M Hill-Burns, Andrew G Clark
Nov 17, 2010·Journal of Molecular Evolution·Vignesh Sundararajan, Alberto Civetta
Jul 7, 2015·Scientific Reports·Suzanne Gomes, Alberto Civetta
Jan 29, 2011·Evolution; International Journal of Organic Evolution·Kenneth M FedorkaBrian Ware
Mar 10, 2016·Genome Biology and Evolution·Richard H BakerGerald S Wilkinson
Aug 23, 2018·Genetics·Ana LlopartJosep M Comeron
Nov 23, 2021·Journal of Molecular Evolution·Rajanikanth Chowdanayaka, Ramachandra Nallur Basappa

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Methods Mentioned

BETA
PCRs
PCR
dissections
Assay

Software Mentioned

BLAST
StatView

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