Gene expression imaging with radiolabeled peptides

Annals of Nuclear Medicine
D J BuchsbaumK R Zinn

Abstract

An approach to image radiolabeled peptide localization at tumor sites by inducing tumor cells to synthesize membrane expressed human somatostatin receptor subtype 2 (hSSTr2) with a high affinity for radiolabeled somatostatin analogues is described. The use of gene transfer technology to induce expression of high affinity membrane hSSTr2 can enhance the specificity and degree of radiolabeled peptide localization in tumors. Employing this strategy, induction of high levels of hSSTr2 expression with selective tumor uptake of radiolabeled peptides was achieved in both subcutaneous non-small cell lung cancer and intraperitoneal ovarian cancer mouse human tumor xenograft models. The features of this genetic transduction imaging approach are: (1) constitutive expression of a tumor-associated receptor is not required; (2) tumor cells are altered to express a new target receptor or increased quantities of a constitutive receptor at levels which may significantly increase tumor targeting of radiolabeled peptides compared to uptake in normal tissues; (3) gene transfer can be accomplished by local or regional injection of adenoviral vectors; (4) it is feasible to target adenovirus vectors to tumor cells by modifying adenoviral tropism (bin...Continue Reading

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Citations

May 6, 2008·Cancer Biotherapy & Radiopharmaceuticals·Monique de VisserMarion de Jong
Jan 4, 2008·ILAR Journal·Kurt R ZinnStuart J Frank
Dec 24, 2005·Expert Opinion on Biological Therapy·Girja S Shukla, David N Krag
Dec 7, 2006·Neuroimaging Clinics of North America·Dawid Schellingerhout
Oct 10, 2013·BioMed Research International·Ling WangHong Zhang
Nov 20, 2016·Cancer Gene Therapy·Z SaadatpourM Keshavarzi

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