Gene expression in adult metafemales of Drosophila melanogaster.

Genetics
J A BirchlerM Krietzman

Abstract

The expression of selected X-linked and autosomal genes was examined in metafemales (3X:2A) compared to diploid sisters. Three enzyme activities (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, beta-hydroxyacid dehydrogenase) encoded by X-linked genes are not significantly different in the two classes of flies. In contrast, three autosomally encoded enzyme activities (alcohol dehydrogenase, alpha-glycerophosphate dehydrogenase, isocitrate dehydrogenase) are reduced in metafemales. Protein and DNA comparisons between metafemales and diploid sisters show a lowered level of total protein whereas the total DNA measurements are similar. Thus, the total cell number in metafemales is basically unchanged but gene expression is reduced. Phenotypic analysis of three autosomal loci, glass (gl), purple (pr) and pink-peach (pp), show that all three have lowered expression in metafemales while the X-linked loci, white-apricot (wa) and Bar (B), are dosage compensated. Quantitative dot blot analysis of messenger RNA levels of the second chromosomal locus, alcohol dehydrogenase (Adh), and the X chromosomal locus, rudimentary (r), show that Adh has reduced expression and r is partially compensated per total RNA in metafemale...Continue Reading

Citations

Feb 6, 2007·Biochimica Et Biophysica Acta·James A BirchlerSiva Chudalayandi
May 16, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·James A BirchlerHarsh H Kavi
Aug 3, 2006·Annual Review of Entomology·Sandra R Schulze, Lori L Wallrath
Nov 23, 2019·Epigenomics·Shihoko Kojima, Daniela Cimini
Feb 19, 2009·Heredity·J A Birchler
Aug 21, 2016·Genes & Development·Lin YangJeannie T Lee
Aug 27, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·James BirchlerAbhijit Sanyal

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