Oct 24, 2013

Gene expression is dynamically regulated in retinal progenitor cells prior to and during overt cellular differentiation

Gene Expression Patterns : GEP
Rajiv DixitCarol Schuurmans

Abstract

The retina is comprised of one glial and six neuronal populations that are generated from a multipotent pool of retinal progenitor cells (RPCs) during development. To give rise to these different cell types, RPCs undergo temporal identity transitions, displaying distinct gene expression profiles at different stages of differentiation. Little, however, is known about temporal differences in RPC identities prior to the onset of overt cellular differentiation, during the period when a retinal identity is gradually acquired. Here we examined the sequential onset of expression of regional markers (i.e., homeodomain transcription factors) and cell fate determinants (i.e., basic-helix-loop-helix transcription factors and neurogenic genes) in RPCs from the earliest appearance of a morphologically-distinct retina. By performing a comparative analysis of the expression of a panel of 27 homeodomain, basic-helix-loop-helix and Notch pathway genes between embryonic day (E) 8.75 and postnatal day (P) 9, we identified six distinct RPC molecular profiles. At E8.75, the earliest stage assayed, murine RPCs expressed five homeodomain genes and a single neurogenic gene (Pax6, Six3, Six6, Rx, Otx2, Hes1). This early gene expression profile was rema...Continue Reading

Mentioned in this Paper

Vertebrates
Photoreceptors
Embryo
NeuroD protein
Amphibians
Biochemical Pathway
Receptors, Notch
Microarray Analysis
BHLHE22 wt Allele
Entire Optic Nerve

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