Gene expression mapping of histone deacetylases and co-factors, and correlation with survival time and 1H-HRMAS metabolomic profile in human gliomas

Scientific Reports
Nassim Dali-YoucefJohan Auwerx

Abstract

Primary brain tumors are presently classified based on imaging and histopathological techniques, which remains unsatisfaying. We profiled here by quantitative real time PCR (qRT-PCR) the transcripts of eighteen histone deacetylases (HDACs) and a subset of transcriptional co-factors in non-tumoral brain samples from 15 patients operated for epilepsia and in brain tumor samples from 50 patients diagnosed with grade II oligodendrogliomas (ODII, n = 9), grade III oligodendrogliomas (ODIII, n = 22) and glioblastomas (GL, n = 19). Co-factor transcripts were significantly different in tumors as compared to non-tumoral samples and distinguished different molecular subgroups of brain tumors, regardless of tumor grade. Among all patients studied, the expression of HDAC1 and HDAC3 was inversely correlated with survival, whereas the expression of HDAC4, HDAC5, HDAC6, HDAC11 and SIRT1 was significantly and positively correlated with survival time of patients with gliomas. (1)H-HRMAS technology revealed metabolomically distinct groups according to the expression of HDAC1, HDAC4 and SIRT1, suggesting that these genes may play an important role in regulating brain tumorigenesis and cancer progression. Our study hence identified different molec...Continue Reading

References

Sep 10, 2003·Biochemical and Biophysical Research Communications·Masaharu HiratsukaMitsuo Oshimura
Feb 24, 1956·Science·O WARBURG
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jung-Hyun ParkYung-Jue Bang
Oct 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zhenhuan ZhangHirotaka Iwase
Sep 17, 2005·Molecular and Cellular Biology·Xuan ZhaoTso-Pang Yao
Sep 7, 2006·Nature Reviews. Drug Discovery·Jessica E BoldenRicky W Johnstone
Mar 24, 2007·Nature Reviews. Molecular Cell Biology·Kenneth K Lee, Jerry L Workman
Aug 19, 2007·Oncogene·M A Glozak, E Seto
Aug 19, 2007·Annals of Medicine·Nassim Dali-YoucefJohan Auwerx
Dec 8, 2007·Epigenetics : Official Journal of the DNA Methylation Society·Ramon Martinez, Gabriele Schackert
Feb 23, 2008·Nature Reviews. Molecular Cell Biology·Xiang-Jiao Yang, Edward Seto
Apr 5, 2008·Science·Kaori IshikawaJun-Ichi Hayashi
Jul 18, 2008·Molecular Biology of the Cell·Andrew J WilsonJohn M Mariadason
Nov 18, 2008·Nature Immunology·Alejandro VillagraEduardo M Sotomayor
Jan 27, 2009·Biochemical and Biophysical Research Communications·Charn-Jung ChangHsin-I Ma
Jun 1, 2007·Molecular Oncology·Santiago Ropero, Manel Esteller
Jun 23, 2009·The Journal of Biological Chemistry·Przemyslaw WareskiAllen Kaasik
May 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joanna D StewartJan G Hengstler
Oct 2, 2012·International Journal of Cancer. Journal International Du Cancer·Hedwig E DeubzerOlaf Witt
May 6, 2014·Cell Metabolism·Laurent MouchiroudJohan Auwerx
May 21, 2014·Neuro-oncology·Quinn T OstromJill S Barnholtz-Sloan

❮ Previous
Next ❯

Citations

Oct 1, 2015·International Journal of Cancer. Journal International Du Cancer·Ulf D KahlertCharles G Eberhart
Sep 29, 2015·International Journal of Oncology·Shiyuan SongGuangping Zhang
May 1, 2019·Metabolomics : Official Journal of the Metabolomic Society·Caroline BundIzzie Jacques Namer
Sep 17, 2019·Clinical Nuclear Medicine·Caroline BundIzzie Jacques Namer
Jun 26, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Tieyi HuYi You
Dec 14, 2017·International Journal of Genomics·Barbara BanelliMassimo Romani
Jul 9, 2020·Cancer Chemotherapy and Pharmacology·R Gajendra ReddyArvind Kumar

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection
surgical resection

Software Mentioned

GraphPad Prism
TMEV
R

Related Concepts

Related Feeds

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.