Gene expression modulation in TGF-β3-mediated rabbit bone marrow stem cells using electrospun scaffolds of various stiffness

Journal of Cellular and Molecular Medicine
Qianping GuoBin Li

Abstract

Tissue engineering has recently evolved into a promising approach for annulus fibrosus (AF) regeneration. However, selection of an ideal cell source, which can be readily differentiated into AF cells of various regions, remains challenging because of the heterogeneity of AF tissue. In this study, we set out to explore the feasibility of using transforming growth factor-β3-mediated bone marrow stem cells (tBMSCs) for AF tissue engineering. Since the differentiation of stem cells significantly relies on the stiffness of substrate, we fabricated nanofibrous scaffolds from a series of biodegradable poly(ether carbonate urethane)-urea (PECUU) materials whose elastic modulus approximated that of native AF tissue. We cultured tBMSCs on PECUU scaffolds and compared their gene expression profile to AF-derived stem cells (AFSCs), the newly identified AF tissue-specific stem cells. As predicted, the expression of collagen-I in both tBMSCs and AFSCs increased with scaffold stiffness, whereas the expression of collagen-II and aggrecan genes showed an opposite trend. Interestingly, the expression of collagen-I, collagen-II and aggrecan genes in tBMSCs on PECUU scaffolds were consistently higher than those in AFSCs regardless of scaffold stif...Continue Reading

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Citations

Feb 17, 2017·Cell Proliferation·Tao ZhangXiaoxiao Cai
Aug 23, 2018·Regenerative Medicine·Lucas Dall AgnolOtávio Bianchi
Sep 5, 2020·Journal of Biomaterials Science. Polymer Edition·Chen LiuHongguang Xu
Jul 26, 2018·Frontiers in Bioengineering and Biotechnology·Genglei ChuBin Li
Oct 30, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xize GaoJun Wu
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Yizhong PengZengwu Shao
Jan 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fengxuan HanBin Li
Sep 11, 2020·Tissue Engineering. Part B, Reviews·Ana P PeredoRobert L Mauck

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Methods Mentioned

BETA
scanning electron microscopy
PCR

Software Mentioned

SPSS
MATLAB
Image J

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