PMID: 7541780Jul 1, 1995Paper

Gene expression of brain nitric oxide synthase and soluble guanylyl cyclase in hypothalamus and medulla of two-kidney, one clip hypertensive rats

Hypertension
T L KrukoffJ Wagner

Abstract

Nitric oxide may act at autonomic sites in the brain to regulate arterial blood pressure. Our goal was to determine whether gene expressions of the brain isoform of nitric oxide synthase and of the beta subunit of soluble guanylyl cyclase, the target of nitric oxide, were altered in discrete autonomic brain regions after induction of hypertension in rats. The two-kidney, one clip model was used to induce hypertension, and measurements were made 3 and 6 weeks after the left renal artery was clipped. Only experimental rats with blood pressures elevated by at least 25 mm Hg were used. Total RNA was purified from microdissected tissue blocks containing hypothalamus, dorsal medulla, rostral ventrolateral medulla, and caudal ventrolateral medulla. Changes in nitric oxide synthase and guanylyl cyclase mRNA were semiquantified in each region by use of reverse transcription-polymerase chain reactions in which known concentrations of deletion mutants of the two genes were coamplified as internal standards. Compared with controls, significant decreases and increases in nitric oxide synthase mRNA were found in the hypothalamus (x 2.2) and caudal ventrolateral medulla (x 6.4), respectively, of hypertensive rats 3 weeks after clipping. These...Continue Reading

References

Apr 25, 1977·Biochemical and Biophysical Research Communications·N MikiK Kuriyama
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W P ArnoldF Murad
Jan 1, 1992·Neuroscience·S R Vincent, H Kimura
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Dec 1, 1991·The American Journal of Physiology·J G WongD E Johnson
May 1, 1991·Hypertension·M Martinez-Maldonado
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M WangD F Mark
Dec 30, 1988·Biochemical and Biophysical Research Communications·M NakaneF Murad
Jan 1, 1988·Annual Review of Physiology·F R Calaresu, C P Yardley
Dec 1, 1980·Journal of Neuroscience Methods·G PaxinosP C Emson
Jan 1, 1983·Annual Review of Neuroscience·L W Swanson, P E Sawchenko
Jan 1, 1994·Annual Review of Neuroscience·E M Schuman, D V Madison
Mar 1, 1994·Neuroscience·N J DunU Förstermann
Sep 16, 1994·Brain Research. Developmental Brain Research·G GiuiliG Guellaën
Feb 1, 1994·The European Journal of Neuroscience·J M Burgunder, P T Cheung
Jan 1, 1994·The American Journal of Physiology·T HornA V Ferguson

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Citations

Jan 27, 1997·The Journal of Comparative Neurology·T L Krukoff, P Khalili
Jan 26, 2002·Microscopy Research and Technique·Yoichi UetaStafford L Lightman
Nov 10, 2001·Brain Research·M L WeissJ Huang
Jul 17, 1999·Brain Research. Brain Research Reviews·T L Krukoff
Oct 23, 1997·Brain Research. Molecular Brain Research·D Plochocka-Zulinska, T L Krukoff
Jul 31, 2001·Autonomic Neuroscience : Basic & Clinical·R SerinoH Yamashita
Jun 1, 2000·Clinical and Experimental Pharmacology & Physiology·M Kadekaro, J Y Summy-Long
Jan 23, 1999·Current Opinion in Nephrology and Hypertension·M Sander, R G Victor
Jan 3, 2013·Clinical and Experimental Pharmacology & Physiology·Megumi Tandai-HirumaYasuhiro Nishida
Jul 23, 1998·Clinical and Experimental Pharmacology & Physiology·T L Krukoff
Apr 28, 2005·Clinical and Experimental Pharmacology & Physiology·Jacqueline K Phillips
May 8, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yoshitaka HirookaAkira Takeshita
Dec 22, 1999·The Japanese Journal of Physiology·M MaedaM Nakai
Mar 19, 2005·Journal of Hypertension·Megumi Tandai-HirumaYasuhiro Nishida
May 2, 2006·Acta Biologica Hungarica·B MornaguiN Gharbi
Jan 24, 2012·Nitric Oxide : Biology and Chemistry·Uberdan Guilherme Mendes de CastroAndréia Carvalho Alzamora
Aug 25, 1997·The Journal of Comparative Neurology·T L KrukoffJ H Jhamandas
Feb 4, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yoshitaka HirookaKenji Sunagawa
Oct 11, 2001·American Journal of Physiology. Renal Physiology·C DechowJ Wagner
Oct 3, 2001·Physiological Reviews·H E de Wardener
Mar 12, 1999·Journal of the American Society of Nephrology : JASN·J WagnerE Ritz

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