Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction

Letters in Applied Microbiology
M KataokaHisashi Kawasaki

Abstract

The ultimate aim is to elucidate the molecular mechanisms for glutamate overproduction by Corynebacterium glutamicum. Gene expression in response to the conditions inducing glutamate overproduction was investigated by using a DNA microarray technique. Most genes involved in the EMP pathway, the PPP, and the TCA cycle were downregulated, while five genes that were highly upregulated (NCgl0917, NCgl2944, NCgl2945, NCgl2946, and NCgl2975) were identified under all the three conditions for overproduction that are studied here. Gene products of NCgl2944, NCgl2945, and NCgl2946 were highly homologous to each other, did not resemble any other protein, and have remained uncharacterized thus far. The product of NCgl0917 showed a similarity to a few hypothetical and uncharacterized proteins. NCgl2975 was homologous to metal-binding proteins. The decrease in the activity of 2-oxoglutarate dehydrogenase complex, a key enzyme that is downregulated during glutamate overproduction, can be mainly attributed to the downregulation of odhA and sucB. Five highly upregulated genes were also identified. Although fermentative production of glutamate has been carried out for more than 45 years, information on the molecular mechanisms of glutamate over...Continue Reading

References

Sep 2, 2003·Journal of Biotechnology·Thomas Hermann
Nov 18, 2004·Journal of Bacteriology·Andrew T UlijaszBernard Weisblum

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Citations

Dec 15, 2010·Bioscience, Biotechnology, and Biochemistry·Ken-ichi HashimotoHisashi Kawasaki
Jan 25, 2011·Prikladnaia biokhimiia i mikrobiologiia·N HaoP K Ouyang
Jul 19, 2013·The Journal of General and Applied Microbiology·Tanyanut SupkulsutraMasaaki Wachi
Feb 26, 2008·Journal of Bioscience and Bioengineering·Takuo HasegawaTsuyoshi Nakamatsu
Aug 13, 2015·Microbial Cell Factories·Minliang ChenYonghua Xiong
Oct 9, 2016·BMC Microbiology·Dorit Lubitz, Volker F Wendisch
Sep 2, 2008·Biological & Pharmaceutical Bulletin·Fumitaka KinugasaSeitaro Mutoh
Sep 6, 2011·Nucleosides, Nucleotides & Nucleic Acids·Mikhail Yu SteklovSergey N Mikhailov

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