Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus

BMC Genomics
Sara de Las Heras-SaldanaCedric Gondro

Abstract

Korean Hanwoo cattle are known for their high meat quality, especially their high intramuscular fat compared to most other cattle breeds. Different muscles have very different meat quality traits and a study of the myogenic process in satellite cells can help us better understand the genes and pathways that regulate this process and how muscles differentiate. Cell cultures of Longissimus dorsi muscle differentiated from myoblast into multinucleated myotubes faster than semimembranosus. Time-series RNA-seq identified a total of 13 differentially expressed genes between the two muscles during their development. These genes seem to be involved in determining muscle lineage development and appear to modulate the expression of myogenic regulatory factors (mainly MYOD and MYF5) during differentiation of satellite cells into multinucleate myotubes. Gene ontology enriched terms were consistent with the morphological changes observed in the histology. Most of the over-represented terms and genes expressed during myoblast differentiation were similar regardless of muscle type which indicates a highly conserved myogenic process albeit the rates of differentiation being different. There were more differences in the enriched GO terms during...Continue Reading

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Citations

May 21, 2020·Veterinary Medicine and Science·Li Yieng LauMarina R S Fortes
Jul 11, 2020·International Journal of Molecular Sciences·Anna CiecierskaTomasz Sadkowski
Nov 30, 2020·Meat Science·Maria Malane Magalhães MunizLucia Galvão de Albuquerque
Dec 3, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Bishi ZhaoWenzhong Liu

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Methods Mentioned

BETA
biopsies
RNA-seq
electrophoresis
PCR

Software Mentioned

package
R
Trimmomatic
Bowtie2
SAS
FastQC
GenomicFeatures
VennDiagram
Ensembl
edgeR

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