PMID: 9545585Apr 18, 1998Paper

Gene expression of mouse M1 and M2 pyruvate kinase isoenzymes correlates with differential poly[A] tract extension of their mRNAs during the development of spermatogenesis

Biochimica Et Biophysica Acta
O de Luis, J del Mazo

Abstract

In eukaryotes, different isoenzymes for pyruvate kinase have been characterized. M2-type Pk cDNA from a mouse fetal ovary library was isolated and differential expression for M1 and M2-types during testis development was observed. While the presence of M2 mRNAs decreases throughout the development of spermatogenesis, we deduced that M1 type expression increases in adult testis coinciding with the presence of elongating spermatids in the seminiferous epithelium. Polyadenylation tests showed a concurrent increase in the length of the polyadenylation tail of transcribed M1-type pyruvate kinase mRNAs in prepuberal to adult seminiferous tubules. A similar relationship between poly[A] tail extension and differential increase of gene expression was detected for M1-type mRNA in adult brain and muscle. Length of poly[A] tail of M2-type transcripts is shown to decrease during the development of mouse testis. These results suggest that changes in the length of the poly[A] tail of transcripts are associated with differential expression of both regulated isoenzymes during testicular development.

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Citations

May 9, 2002·Molecular and Cellular Endocrinology·T KuraishiS Sakai
Aug 6, 2004·DNA and Cell Biology·M AnithaR Bamezai
Oct 15, 2010·Comparative Hepatology·Elke UeberhamRolf Gebhardt
Jul 8, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yusuke EchigoyaTakeo Sakai
Jan 15, 2008·The International Journal of Biochemistry & Cell Biology·Jungwoon LeeJungho Kim
May 12, 2006·Biology of Reproduction·Michelle KrisfalusiDeborah A O'Brien
May 20, 2003·Molecular & Cellular Proteomics : MCP·Emmanuelle ComCharles Pineau
Aug 2, 2008·Reproduction, Fertility, and Development·Sandra FeidenGünter Kamp
Aug 12, 2019·Free Radical Biology & Medicine·Mohammed AlquraishiAhmed Bettaieb

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