Gene expression of muscarinic, tachykinin, and purinergic receptors in porcine bladder: comparison with cultured cells

Frontiers in Pharmacology
Forough BahadoryE Burcher

Abstract

Urothelial cells, myofibroblasts, and smooth muscle cells are important cell types contributing to bladder function. Multiple receptors including muscarinic (M3/M5), tachykinin (NK1/NK2), and purinergic (P2X1/P2Y6) receptors are involved in bladder motor and sensory actions. Using female pig bladder, our aim was to differentiate between various cell types in bladder by genetic markers. We compared the molecular expression pattern between the fresh tissue layers and their cultured cell counterparts. We also examined responses to agonists for these receptors in cultured cells. Urothelial, suburothelial (myofibroblasts), and smooth muscle cells isolated from pig bladder were cultured (10-14 days) and identified by marker antibodies. Gene (mRNA) expression level was demonstrated by real-time PCR. The receptor expression pattern was very similar between suburothelium and detrusor, and higher than urothelium. The gene expression of all receptors decreased in culture compared with the fresh tissue, although the reduction in cultured urothelial cells appeared less significant compared to suburothelial and detrusor cells. Cultured myofibroblasts and detrusor cells did not contract in response to the agonists acetylcholine, neurokinin A,...Continue Reading

Citations

Feb 24, 2015·Expert Opinion on Therapeutic Targets·Martin C Michel, Yasuhiko Igawa
Sep 25, 2014·Annual Review of Pharmacology and Toxicology·Martin C Michel
Mar 4, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hafiz Sana-Ur-RehmanLu Liu
May 29, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Donna SellersMartin C Michel

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Methods Mentioned

BETA
PCR

Software Mentioned

Primer
ImageJ

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