Abstract
Most previous researches on neurotrophins including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) have focused on the nervous system, because their receptors are widely distributed in neuronal tissues. Recently, however, the participation of neurotrophins in inflammation and atherosclerosis has been proposed. Therefore, the gene expression of neurotrophins is now an urgent issue is to be investigated in nonneuronal tissues. Here, we evaluated the gene expression of neurotrophins and their receptors in rat cultured vascular smooth muscle cells (VSMCs) by the reverse transcriptase-polymerase chain reaction method. The transcripts of NGF, NT-3, and TrkC (high-affinity receptor for NT-3), and two BDNF alternative spliced transcript variants with exons 3 and 4 were clearly detected in VSMCs cultured under conventional culture conditions. The upregulation of mRNA levels for NGF, two BDNF variants with exons 1 and 2, low-affinity neurotrophin receptor, and high-affinity receptors, TrkA (for NGF) and TrkB (for BDNF), was observed in response to the treatment with serum and phorbol-ester following the serum-starvation. In contrast, the expression of NT-3 and TrkC genes was downregulated u...Continue Reading
References
Jan 1, 1979·Physiological Reviews·J Chamley-CampbellR Ross
Nov 16, 1992·Gene·M MetsisH Persson
Oct 1, 1992·The Journal of Cell Biology·M MeyerH Thoenen
Feb 1, 1992·The Journal of Clinical Investigation·A JawienA W Clowes
Jul 12, 1991·Cell·C Cordon-CardoL F Reichardt
Nov 1, 1991·Neuron·L R BerkemeierA Rosenthal
Dec 1, 1991·Hypertension·D Creedon, J B Tuttle
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B HengererH Thoenen
Mar 22, 1990·Nature·A HohnY A Barde
Mar 12, 1990·FEBS Letters·D WionP Brachet
Aug 18, 1988·Nature·W W LamphI M Verma
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I MocchettiE Costa
Sep 14, 1989·Nature·J LeibrockY A Barde
Feb 12, 1987·Nature·M J RadekeE M Shooter
Aug 12, 1988·Cell·P Sassone-CorsiI M Verma
Sep 1, 1987·Molecular and Cellular Biology·M J SelbyW J Rutter
Apr 1, 1985·Experimental and Molecular Pathology·G R Campbell, J H Campbell
Apr 7, 1983·Nature·J ScottW J Rutter
Nov 1, 1981·Atherosclerosis·J H Chamley-Campbell, G R Campbell
Dec 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M CartwrightG Heinrich
Nov 1, 1994·Journal of Neurobiology·M V Chao
Nov 1, 1994·Journal of Neurobiology·M Barbacid
Oct 15, 1994·Journal of Neuroscience Research·E OmaeS Furukawa
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P B EhrhardU Otten
Jul 1, 1994·The European Journal of Neuroscience·A Leingärtner, D Lindholm
Feb 1, 1994·Brain Research. Molecular Brain Research·M NakayamaO Ohara
Sep 10, 1993·Brain Research·X F Zhou, R A Rush
Nov 1, 1993·Brain Research. Molecular Brain Research·T NeumanP Gruss
Jan 1, 1993·Brain Research. Molecular Brain Research·A ShintaniK Igarashi
Mar 1, 1993·Neuron·T TimmuskH Persson
Apr 29, 1993·Nature·R Ross
May 3, 1996·Cell·M TorciaE Garaci
Oct 1, 1996·Nature Genetics·M J DonovanB L Hempstead
Nov 1, 1996·Circulation Research·J O HiltunenM Saarma
Dec 13, 1996·Biochemical and Biophysical Research Communications·H PoukkaJ J Palvimo
May 1, 1997·Brain Research. Molecular Brain Research·V Y HayesP J Isackson
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·L TessarolloL F Parada
Feb 7, 1998·The Journal of Biological Chemistry·B B ChangD L Kilpatrick
Citations
Apr 11, 2000·Journal of Neurotrauma·M HayashiE Senba
Jan 8, 2009·Physiological Reviews·Andrea Caporali, Costanza Emanueli
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Jenny TolletMalcolm P Sparrow
Sep 15, 2005·BMC Neuroscience·Vanessa L NordellFarida Sohrabji
Jul 20, 2005·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Kiyomitsu NemotoTakashi Ueyama
Feb 6, 2014·PloS One·Agustin AnastasiaPouneh Kermani
Jan 29, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A BraunH Renz
Aug 13, 2010·Psychoneuroendocrinology·B A A BusR C Oude Voshaar
Mar 19, 2008·International Journal of Oral and Maxillofacial Surgery·J-H ByunB-W Park
Oct 5, 2007·Pharmacology & Therapeutics·V Freund-Michel, N Frossard
Jul 14, 2007·Life Sciences·Haruko Kawaguchi-ManabeKeiichi Fukuda
May 8, 2007·Trends in Cardiovascular Medicine·Pouneh Kermani, Barbara Hempstead
Aug 20, 2003·The European Journal of Neuroscience·Yutaka KoyamaAkemichi Baba
Oct 7, 2004·European Journal of Pharmacology·Nelly FrossardCharles Advenier
Jun 15, 2004·Biochemical Pharmacology·Yutaka KoyamaToshio Matsuda
Jan 21, 2012·Microvascular Research·Tongrong He, Zvonimir S Katusic
Jun 10, 2006·Anatomy and Embryology·O García-SuárezJ A Vega
Jun 17, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T YamashiroT Takano-Yamamoto
Mar 21, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Christine MarieCéline Demougeot
Mar 29, 2000·FEBS Letters·T NakahashiB Sun
Apr 29, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Chunyan SunLu Zhang
Jul 7, 2007·Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·S S M RensenG J J M van Eys
Feb 14, 2006·Blood·Eleanor ChenStephen C Ekker
Oct 16, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·T LumG Heinrich
Feb 26, 2010·Endocrinology·Mária SzekeresLászló Hunyady
May 21, 2019·Frontiers in Physiology·Pouneh Kermani, Barbara Hempstead
Mar 6, 2021·Journal of the American Heart Association·Sarah ZieroldKatrin Schäfer
Aug 31, 2000·Biochemical and Biophysical Research Communications·K NemotoM Degawa
May 5, 2001·Bone·T YamashiroT Takano-Yamamoto
Jun 24, 2004·Revue des maladies respiratoires·V Freund, N Frossard