Gene expression of prothrombin in the human kidney and its potential relevance to kidney stone disease

British Journal of Urology
A M StapletonR L Ryall

Abstract

To determine whether urinary prothrombin fragment 1 (UPTF1), which shows considerable promise as a critical determinant of calcium oxalate (CaOx) stone formation, is manufactured by the human kidney. Ribonucleic acid was isolated from eight kidneys, two spleens and one liver. Using reverse transcriptase-polymerase chain reaction, mRNA corresponding to the UPTF1 portion of prothrombin was analysed by agarose-gel electrophoresis and Southern blotting. Six kidney specimens showed clear evidence of prothrombin gene expression; expression in the kidney was less than that in the liver. This is the first demonstration of prothrombin gene expression within the human kidney, a finding that not only has implications for CaOx stone disease but also potentially for blood coagulation.

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Citations

Dec 22, 1999·European Journal of Biochemistry·P K GroverR L Ryall
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N P BuchholzR L Ryall
Oct 24, 2013·BioMed Research International·Kanu Priya AggarwalChanderdeep Tandon
Feb 13, 2001·Biochemical and Biophysical Research Communications·L M StenbergJ Stenflo
Jan 10, 2001·Current Opinion in Urology·C F Verkoelen, M S Schepers
Aug 21, 2009·The American Journal of Clinical Nutrition·Joyce C McCann, Bruce N Ames
Apr 20, 2006·American Journal of Physiology. Renal Physiology·Kristin J BergslandFredric L Coe

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