Abstract
To determine whether urinary prothrombin fragment 1 (UPTF1), which shows considerable promise as a critical determinant of calcium oxalate (CaOx) stone formation, is manufactured by the human kidney. Ribonucleic acid was isolated from eight kidneys, two spleens and one liver. Using reverse transcriptase-polymerase chain reaction, mRNA corresponding to the UPTF1 portion of prothrombin was analysed by agarose-gel electrophoresis and Southern blotting. Six kidney specimens showed clear evidence of prothrombin gene expression; expression in the kidney was less than that in the liver. This is the first demonstration of prothrombin gene expression within the human kidney, a finding that not only has implications for CaOx stone disease but also potentially for blood coagulation.
References
Aug 23, 1976·Biochemical and Biophysical Research Communications·P V HauschkaP M Gallop
May 1, 1978·Kidney International·H Fleisch
Jul 1, 1992·The Journal of Cell Biology·K Jalink, W H Moolenaar
Jan 1, 1992·Seminars in Thrombosis and Hemostasis·S J Degen
Feb 16, 1991·Biochimica Et Biophysica Acta·C S Jamison, S J Degen
Oct 29, 1991·Biochemistry·E W DavieW Kisiel
Apr 1, 1991·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·F L CoeJ H Parks
Apr 1, 1991·Neuron·M DihanichD Monard
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D Gurwitz, D D Cunningham
Mar 11, 1986·Biochemistry·R W RombergK G Mann
Dec 5, 1973·Biochimica Et Biophysica Acta·W Kisiel, D J Hanahan
Sep 25, 1981·Molecular and Cellular Biochemistry·J P BurnierB Furie
Dec 1, 1984·British Journal of Haematology·A Bezeaud, M C Guillin
Oct 1, 1993·Kidney International·A M StapletonR L Ryall
Jun 1, 1995·British Journal of Urology·A M Stapleton, R L Ryall
Sep 30, 1993·Biochemical and Biophysical Research Communications·A M StapletonR L Ryall
Oct 1, 1993·Trends in Pharmacological Sciences·C TapparelliN S Cook
Nov 1, 1995·Clinical Science·R L RyallR J Simpson
Mar 1, 1996·Kidney International·A M StapletonR L Ryall
Citations
Dec 4, 2003·European Urology·A HesseP Alken
May 5, 2001·The Biochemical Journal·P K GroverR L Ryall
Aug 3, 1999·European Journal of Biochemistry·P K Grover, R L Ryall
Dec 22, 1999·European Journal of Biochemistry·P K GroverR L Ryall
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N P BuchholzR L Ryall
Oct 24, 2013·BioMed Research International·Kanu Priya AggarwalChanderdeep Tandon
Feb 13, 2001·Biochemical and Biophysical Research Communications·L M StenbergJ Stenflo
Nov 22, 2008·BJU International·Alison F CookRosemary L Ryall
Jan 18, 2006·Urology·Jihong LiuZhangqun Ye
Jan 10, 2001·Current Opinion in Urology·C F Verkoelen, M S Schepers
Sep 19, 2006·The Journal of Pathology·P K GroverR L Ryall
Nov 2, 2017·PloS One·Sunil JoshiSaeed R Khan
Aug 21, 2009·The American Journal of Clinical Nutrition·Joyce C McCann, Bruce N Ames
Aug 10, 2013·The Journal of Biological Chemistry·Abdessalem HammedVirginie Lattard
Oct 28, 2000·The Journal of Biological Chemistry·J van AdelsbergY Huan
Apr 20, 2006·American Journal of Physiology. Renal Physiology·Kristin J BergslandFredric L Coe