Gene expression of Vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment

BMC Microbiology
Katherine GarcíaRomilio T Espejo

Abstract

Vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °C, a condition seldom found in the ocean. However, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. In this context, revealing how gene expression enables V. parahaemolyticus to adapt to this particular condition -common to almost all V. parahaemolyticus isolates- will improve our understanding of the biology of this important pathogen. To determine the genes of V. parahaemolyticus differentially expressed when growing in isolation condition (37 °C, 0.9% NaCl, and 0.04% bile salts) referred to those at the temperature and salt concentration prevailing in ocean south of Chile (marine-like condition; 12 °C, 3% NaCl, and absence of bile salts) we used high-throughput sequencing of RNA. Our results showed that in the isolation condition, among the 5034 genes annotated in the V. parahaemolyticus RIMD2210633 genome, 344 were upregulated and 433 downregulated referred to the marine-like condition, managing an adjusted P-value (Padj) < E-5. Between the 50 more highly expressed genes, among the small RNAs (sRNA), the three carbon s...Continue Reading

References

Jul 13, 2000·Cell·K M Wassarman, G Storz
Apr 25, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K Yamanaka, M Inouye
Aug 24, 2002·Journal of Bacteriology·Kazushi SuzukiTony Romeo
Nov 7, 2003·Applied and Environmental Microbiology·Partha Pratim Datta, Rupak K Bhadra
Feb 12, 2005·Emerging Infectious Diseases·Narjol González-EscalonaRomilio T Espejo
Oct 31, 2007·Advances in Experimental Medicine and Biology·Brian W WorthamMarcos A Oliveira
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jul 26, 2011·Microbes and Infection·Christopher A BrobergKim Orth
Aug 17, 2011·Cell Host & Microbe·Anjali MandlikMatthew K Waldor
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Dec 4, 2012·Nucleic Acids Research·Lei LiHoi Shan Kwan
Nov 12, 2013·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Dec 18, 2013·Cell Host & Microbe·Brian T HoJohn J Mekalanos
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Apr 19, 2015·Veterinary Microbiology·Gaëlle Porcheron, Charles M Dozois
Jan 27, 2017·Frontiers in Microbiology·Diliana Pérez-ReytorKatherine Garcia

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Citations

Apr 15, 2020·Journal of Microbiological Methods·Taro YonekitaTakashi Matsumoto

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Methods Mentioned

BETA
electrophoresis
PCR
Chip
RNA-Seq

Software Mentioned

clusterProfiler
R
count
RefSeq
SAMtools
ShortRead Bioconductor
BWA
MagJET
EnrichGO
BWA )

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